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pTwist-SARS-CoV-2 Δ18 B.1.351v1

Plasmid

#169462

Purpose

Encodes SARS-CoV-2 B.1.351v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Publication

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v1 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

Plasmid

#169469

Purpose

Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Publication

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GW

Plasmid

#27230

Depositor

Pavel Osten

Publication

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

Plasmid

#126679

Purpose

Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

Depositor

Hee Won Yang

Publication

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

Rb (a.a 886-928)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDONR221_SLC3A2

Plasmid

#132213

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Expression

Mammalian

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Publication

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Tags

flag

Expression

Mammalian

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pH2B-miRFP670nano

Plasmid

#127438

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Publication

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-miRFP670nano (H2BC11 Synthetic, Human)

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Halo-MTS

Plasmid

#124315

Purpose

Expresses mitochondria specific HaloTag protein

Depositor

Jin Wang

Publication

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Halo tag

Tags

Mito specific seq

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Publication

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Publication

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

7a sgRNA

Use

CRISPR

Expression

Mammalian

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pCas9-CtIP

Plasmid

#109403

Purpose

Expresses Cas9 fusion to human CtIP protein

Depositor

Jean-Paul Concordet

Publication

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CtIP (RBBP8 Human)

Mutation

full length CtIP

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYLCRISPR/Cas9P35S-H

Plasmid

#66189

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDicAID_nCas9-PmCDA_NptII_Della

Plasmid

#91694

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-I-PhoCl-HCVp

Plasmid

#87697

Purpose

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

Depositor

Robert Campbell

Publication

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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