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pDM1038

Plasmid

#108988

Purpose

Inducible expression vector (dox-on)

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMT445

Plasmid

#139386

Purpose

aTC inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

aTC inducible Nanoluc expression

Use

Synthetic Biology

Promoter

aTC inducible promoter

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDM1047

Plasmid

#108990

Purpose

Inducible expression vector (dox-on), N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGL0_1 [RK2 ORI]

Plasmid

#198918

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RK2 ORI

Use

Synthetic Biology

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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UPmCh

Plasmid

#161004

Purpose

ubiquitous expression of PCP-mCherry

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PCP-mCherry

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RANBP1_Ran-BP1

Plasmid

#109872

Purpose

Protein expression and purification of RANBP1_Ran-BP1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RANBP1_Ran-BP1 (RANBP1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_TCF7L2_WT

Plasmid

#81769

Purpose

Gateway Donor vector containing TCF7L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TCF7L2 (TCF7L2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDK4_WT

Plasmid

#81741

Purpose

Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDK4 (CDK4 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_JAZF1_WT

Plasmid

#82136

Purpose

Gateway Donor vector containing JAZF1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAZF1 (JAZF1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_YFP

Plasmid

#45278

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

YFP

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
EF1a-mCHERRY

Plasmid

#224298

Purpose

Expresses mCherry under EF1a promoter (CTRL vector)

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher

Plasmid

#223633

Purpose

Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B3

Plasmid

#172844

Purpose

CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mTurquoise2

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAI1_G-alpha

Plasmid

#110012

Purpose

Protein expression and purification of GNAI1_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI1_G-alpha (GNAI1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GST-RNF126

Plasmid

#138643

Purpose

Bacterial expression of GST-RNF126 fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRger2-TS-EYFP

Plasmid

#127238

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger2-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

CamKIIa

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRger3-TS-EYFP

Plasmid

#127240

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

hSyn1

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(1,20,6)-ADAR2

Plasmid

#124620

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCIneo-NHA-LacZ

Plasmid

#115363

Purpose

Expression vector to produce NHA-tagged LacZ protein

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

lacZ beta-D-galactosidase

Tags

N peptide and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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