We narrowed to 11,691 results for: NSI
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Plasmid#195897PurposeLevel 0 tUBQ1 terminator with GG4 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES (M)
Plasmid#182437PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD415
Plasmid#178197PurposeRok _Linker overexpressionDepositorInsertrok _linker
ExpressionBacterialMutationdeletion of residues 75-96PromoterT7Available SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD461
Plasmid#178198PurposeRok with a C-terminal His-tag overexpressionDepositorInsertrok 6xHis
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mCherry-HRasCT
Plasmid#186704PurposeEncoding membrane-targeted mCherryDepositorInsertmCherry-HRasCT
ExpressionMammalianAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)
Plasmid#191176PurposeMammalian expression of EK0209 with a second stop codon.DepositorInsertCMV-TO:mCherry:s70587:EGFP (double-stop)
ExpressionMammalianMutationadditional stop codonPromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191178PurposeMammalian expression of EK0493 with second stop codon.DepositorInsertmCherry:GAPDH.s1:EGFP (double-stop)
ExpressionMammalianMutationadditional stop codonPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)
Plasmid#191158PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp longDepositorInsertmCherry:Bdnf.s1:Cre
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)
Plasmid#191166PurposeMammalian expression of a EGFP mutant N213D derived from EK0379.DepositorInsertEGFP(N213D)
ExpressionMammalianMutationc.637A>G(N213D; CCA>CCG)PromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA
Plasmid#191148PurposeAAV expression of a Cre reporter with EYFP outputDepositorInsertDIO-EYFP
UseAAVExpressionMammalianMutationWTPromoterhEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0362 SFFV-mCherry-SG(Bdnf.s1L36+54@202)SG-EGFP (FLP-IN)
Plasmid#191153PurposeMammalian expression of a 90 bp (36+54) long sensor for murine Bdnf 3' UTR where the trigger sequence is splitDepositorInsertmCherry:Bdnf.s1L36+54:EGFP
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS
Plasmid#191365PurposeOverexpression of NLS-dPbaCas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPbaCas13b-EGFP-NLS
TagsEGFPExpressionMammalianAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS
Plasmid#191364PurposeOverexpression of NLS-dPba5Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba5Cas13b-EGFP-NLS
TagsEGFPExpressionMammalianAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS
Plasmid#191363PurposeOverexpression of NLS-dPba4Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba4Cas13b-EGFP-NLS
TagsEGFPExpressionMammalianAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS
Plasmid#191366PurposeOverexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dHgm6Cas13b-EGFP-NLS
TagsEGFPExpressionMammalianAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS
Plasmid#191362PurposeOverexpression of NLS-dBba2Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dBba2Cas13b-EGFP-NLS
TagsEGFPExpressionMammalianAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS
Plasmid#191361PurposeOverexpression of NLS-dPba3Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba3Cas13b-EGFP-NLS
TagsEGFPExpressionMammalianAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14
Plasmid#183062PurposePlasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastPromoterHREsYGP1p and RET2Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9339 (pgRNA_VII-1_NatMX)
Plasmid#161591PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only