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pLV-EF1a-V5-LIC

Plasmid

#120247

Purpose

For LIC cloning with N-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0826-mCherryBb

Plasmid

#118242

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0826 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0826 promoter driven mCherry

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0026-mCherryBb

Plasmid

#118239

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0026 promoter driven mCherry

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-PXN

Plasmid

#112957

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

PXN

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP3

Plasmid

#80659

Purpose

Encodes the synthetic coiled coil peptide SYNZIP3

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP3

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pKM343

Plasmid

#71487

Purpose

Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-cat-loxP

Use

Source of loxp-hyg-cat- loxp for pcr amplificatio…

Expression

Bacterial

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-230

Plasmid

#90499

Purpose

pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tFD and mito-tFNR

Use

Lentiviral

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-202

Plasmid

#90492

Purpose

Expresses pcDNA3 - CMVmin - 5X UAS - pFR - CMVmin Luciferase

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

CMVmin Luciferase Fluc

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pME-mKate2 no stop

Plasmid

#80806

Purpose

red fluorophore without stop codon

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-mKate2 no stop

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMB41

Plasmid

#73087

Purpose

N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

Avi-GFP tag

Tags

Avi and GFP

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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NM-01

Plasmid

#69799

Purpose

Bacterial expression of Cas9 with His, MBP, and Strep tags

Depositor

Gerard Cagney

Insert

Cas9

Tags

HIS, MBP, and StrepII

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR-RNF40i1

Plasmid

#59594

Purpose

Expression of shRNA against human RNF40

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

RNF40 (RNF40 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
AP-p003

Plasmid

#31494

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

Half genome of WU polyomavirus

Expression

Bacterial

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pBM5218

Plasmid

#18796

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Ty5-HIS3AI

Expression

Yeast

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
SGL40C.EFS.E2Crimson

Plasmid

#100894

Purpose

Lentiviral gRNA delivery

Depositor

Dirk Heckl

Article

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Insert

E2Crimson

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-HaloGFP-Na2.4

Plasmid

#228471

Purpose

Mammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGM227

Plasmid

#220182

Purpose

Expresses exogenous wild-type TOMM70A

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM70A (TOMM70 Human)

Use

Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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KW78_HART_ODC1_PuroR

Plasmid

#207401

Purpose

Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR - PuroR

Depositor

Stephen Floor

Article

Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

mini CMV

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-WHaloCaMP1a-EGFP

Plasmid

#205307

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-TOMM20-QUE37C

Plasmid

#191525

Purpose

ATP measurement, mitochondria outer membrane

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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