We narrowed to 15,073 results for: nts

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pMK435

Plasmid

#236573

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-3

Plasmid

#27289

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin beta 3 (ITGB3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-control

Plasmid

#238257

Purpose

For overexpression of EGFP-control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAGM55261

Plasmid

#153210

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-KS

Plasmid

#238233

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_B2520

Plasmid

#91077

Purpose

Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE687

Plasmid

#167299

Purpose

Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR5A L3-L2

Plasmid

#158732

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF7A L1-L4

Plasmid

#158730

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NI)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3A-BE5-NG-BlastR

Plasmid

#152998

Purpose

High purity C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB544

Plasmid

#74878

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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CHMP6_GFP

Plasmid

#31806

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP6 (CHMP6 Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pAGM55273

Plasmid

#153211

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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