We narrowed to 9,675 results for: Ada;

1. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

   Plasmid

   #170163

   Purpose

   Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2s-P2A-H2B-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

   Plasmid

   #90332

   Purpose

   CRISPR interference

   Depositor

   Dirk Heckl

   Article

   Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

   Inserts

   dCas9
   na
   SFFV
   P2A-BSD
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-TCF21

   Plasmid

   #192894

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of TCF21

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   TCF21 (TCF21 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast HA-POLE2

   Plasmid

   #160809

   Purpose

   Express HA-tagged POLE2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   HA-POLE2 (POLE2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT/TO-Ufd1-Strep-HA

   Plasmid

   #113474

   Purpose

   Expression of human Ufd1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Ufd1 (UFD1 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-N-Solo-mStayGold-CENPA

   Plasmid

   #227283

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Integrin beta5-2XEGFP

   Plasmid

   #139779

   Purpose

   Detecting Integrin beta5 by fluorescence microscopy

   Depositor

   Staffan Stromblad

   Article

   Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

   Insert

   Integrin subunit beta5 (ITGB5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

   Plasmid

   #178528

   Purpose

   Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   rtTA, SspB-mScarlet-I-MYPT (1-169aa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, Tet responsive element

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. WDV-Echistatin

   Plasmid

   #226985

   Purpose

   Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

   Depositor

   Wendy Gordon

   Article

   Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

   Inserts

   WDV (repA )
   Echistatin
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNOC_hfnCas12a_CRATES

    Plasmid

    #176251

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFP-mTet1s pc3901

    Plasmid

    #201700

    Purpose

    Expresses of GFP-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1-dmito-oROS-HT

    Plasmid

    #216418

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    dmito-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMK265 (RAD21-mAC Hygro)

    Plasmid

    #140539

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-cFos-XRI-V5

    Plasmid

    #178058

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-V5
    

    Use

    AAV

    Tags

    V5-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse c-fos promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-PITCh-COX8A

    Plasmid

    #227308

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of COX8A (COX8A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U67

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid

    #218508

    Purpose

    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    mNeongreen
    

    Use

    Tags

    modified ssrA degradation tag

    Expression

    Bacterial

    Mutation

    deletion of MCS containing Plac_lacZa, introducti…

    Promoter

    P23

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH-EFIRES-P-AtAFB2-mCherry-weak NLS

    Plasmid

    #129717

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry-weak NLS

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-BHLHE40

    Plasmid

    #110154

    Purpose

    Expression of human BHLHE40 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

    Insert

    BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-neo-RL-ZEB2 200bmut

    Plasmid

    #35538

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB2 gene (ZEB2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    6 Mutations in the miR-200b seed regions of the Z…

    Promoter

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC1-oROS-HT-CaaX

    Plasmid

    #216417

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT-CaaX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge