We narrowed to 58,802 results for: Ran

1. p CIneo-RL-Let7-perf

   Plasmid

   #115367

   Purpose

   Expression vector to produce humanized Renilla luciferase with a single perfectly complementary binding site for human let-7 miRNA in the 3'UTR

   Depositor

   Witold Filipowicz, Ramesh Pillai

   Article

   Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK506

   Plasmid

   #155382

   Purpose

   dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDrop

   Plasmid

   #184872

   Purpose

   Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAWH

   Plasmid

   #133894

   Purpose

   Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBKWH

   Plasmid

   #133898

   Purpose

   Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pColE1_sgRNA_M1S_AmpR

   Plasmid

   #119153

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
   

   Use

   Crispr

   Promoter

   J23119

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pColE1_sgRNA_M1NS_AmpR

   Plasmid

   #119155

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
   

   Use

   Crispr

   Promoter

   J23119

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTetQCas-BsaI

   Plasmid

   #170629

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCasTns(Vch)-entry(BsaI)

   Plasmid

   #190273

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
   

   Expression

   Bacterial

   Mutation

   no

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDDGFP-Leu2d-ScVrg4

    Plasmid

    #102334

    Purpose

    Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

    Insert

    YGL225W (VRG4 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGG-PM-TurboID

    Plasmid

    #209395

    Purpose

    Transiently expressing TurboID fused with a plasma menbrane targeting peptide in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    PM-3XHA-TurbOID
    

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. RP2-EGFP

    Plasmid

    #86073

    Purpose

    A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV/EF1a-DIO-SCLM

    Plasmid

    #216759

    Purpose

    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    EF1a (human elongation factor 1 alpha)

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. rTetR-GFP-VPR

    Plasmid

    #183918

    Purpose

    VPR activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    rTetR-GFP-VPR
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAW2-Smed-Slit-1

    Plasmid

    #182264

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

    Depositor

    Daniel Lobo

    Article

    Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

    Insert

    Smed-Slit-1
    

    Use

    Gibson cloning vector for synthesis of single and…

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

    Plasmid

    #191675

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6X his and flag

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2_VP1_RaPID_r1c3

    Plasmid

    #65723

    Purpose

    Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only

    Depositor

    Junghae Suh

    Article

    Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

    Insert

    mCherry
    

    Use

    AAV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLV GG hUbV-EGFP

    Plasmid

    #216161

    Purpose

    Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRhaQCas-BsaI

    Plasmid

    #170631

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Expression

    Bacterial

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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