161,152 results

1. pCAGG>nls-hCas9-nls-GFP

   Plasmid

   #99141

   Purpose

   Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   Cas9-GFP
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-lox-GFP shRNA p19-2

   Plasmid

   #14091

   Depositor

   Tyler Jacks

   Article

   Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

   Insert

   p19 ARF shRNA -2 (Cdkn2a Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pLenti-Lifeact-tdTomato

   Plasmid

   #64048

   Purpose

   Fluorescent reporter for F-actin labeling in living cells

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet-H_Giantin_C1

   Plasmid

   #85049

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Giantin (GOLGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 3131-3259

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)Gα15-244-NLuc

   Plasmid

   #207154

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA15 (GNA15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position E(244)N

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT/SVNeo

   Plasmid

   #24550

   Depositor

   Perry Hackett

   Article

   Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

   Insert

   pT/SVNeo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pUCM-AAVS1-TO-hNGN2

   Plasmid

   #105840

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mutation

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUW-tetO-Myb

   Plasmid

   #139826

   Purpose

   doxycycline-inducible expression of mouse Myb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Myb (Myb Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P94H and Q619R- Please see depositor comment belo…

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Use

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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