We narrowed to 7,668 results for: CCH

1. pILGFP91

   Plasmid

   #83548

   Purpose

   Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PGK1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

   Plasmid

   #86413

   Purpose

   nuclear split GFP11 reporter with mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   Nop1pr-GFP11-mCherry-PUS1
   

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-LifeAct-FRB-Venus-173-N

   Plasmid

   #87616

   Purpose

   FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-FRB-Venus173-N
   

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pfa6a mScarlet-TsCC(B)::KANMX

   Plasmid

   #176397

   Purpose

   Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   mScarlet-TsCC(B)
   

   Tags

   mScarlet fused to TsCC(B)

   Expression

   Bacterial and Yeast

   Promoter

   none

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

   Plasmid

   #86416

   Purpose

   ONM/ER split GFP11 reporter with mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP11-mCherry-Scs2TM
   

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLOX-CWBmi1

   Plasmid

   #12240

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   Bmi1 (Bmi1 Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   CMV promoter upstream of mBmi-1. LoxP in 3' …

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

   Plasmid

   #86417

   Purpose

   luminal split GFP11 reporter with mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-mCherry-SCS2TM-GFP11
   

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIP sHRPa-Aga1P

   Plasmid

   #73151

   Purpose

   Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-Aga1P
   

   Tags

   V5 tag

   Expression

   Yeast

   Promoter

   glyceraldehyde-3-phosphate dehydrogenase GPD prom…

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

   Plasmid

   #86414

   Purpose

   nuclear split GFP11 reporter without mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP11-PUS1
   

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Promoter

   NOP1pr

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-AKAR4-NLS

    Plasmid

    #138217

    Purpose

    Nuclear-targeted FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    AKAR4-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Plasmid EUPp_09

    Plasmid

    #157745

    Purpose

    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

    Depositor

    Kang Zhou

    Article

    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

    Inserts

    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    PgyrA

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCTCON2 Aga2P-HRP

    Plasmid

    #73152

    Purpose

    Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Aga2P-HRP
    

    Tags

    HA tag and myc tag

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-p62-Silent_Mutation_WT

    Plasmid

    #187986

    Purpose

    human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutation for resistance to sip62 #5

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TRE-FLEX-eNpHR-EYFP

    Plasmid

    #89876

    Purpose

    To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    eNpHR=EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEYFP-Mitotrap

    Plasmid

    #46942

    Purpose

    Expresses Mitotrap in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Mitotrap (MTOR Aequorea victoria, Synthetic, Human, Budding Yeast)
    

    Tags

    This is a fusion between a yeast mitochondrial ta…

    Expression

    Mammalian

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5 alpha 1A AR

    Plasmid

    #45760

    Depositor

    Robert Lefkowitz

    Article

    Schwinn et al J Biol Chem. 1990 May 15;265(14):8183-9.

    Insert

    alpha 1A AR (Adra1a Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pvimentin-emiRFP670

    Plasmid

    #136572

    Purpose

    Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    vimentin-emiRFP670
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-ER-AKAR4

    Plasmid

    #64733

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    AKAR4
    

    Tags

    Cerulean, N-terminal 27 amino acids from CYP450, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYN2_1

    Plasmid

    #184757

    Purpose

    Plasmid for genome editing by CRISPR/Cas9

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    

    Use

    Synthetic Biology

    Tags

    2xNLS

    Expression

    Bacterial and Yeast

    Promoter

    PGK1 and tRNA-Phe-HDV Ribozyme

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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