We narrowed to 10,393 results for: plasmids 101

1. pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

   Plasmid

   #203838

   Purpose

   Flp-dependent Cre and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   mScarlet-KASH-2A

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFGC5941-PacI-ath-STTM165/166-48nt

   Plasmid

   #84091

   Purpose

   target miRNA165/166 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM165/166
   

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-FLEX-EGFP-mir30(Scn9a)

   Plasmid

   #79672

   Purpose

   Cre-dependent knockdown of Scn9a (Nav1.7)

   Depositor

   Scott Sternson

   Article

   Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

   Insert

   EGFP (Scn9a Mouse, jellyfish)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD

   Plasmid

   #188374

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEX-SaCas9-U6-sgSlc18a2

   Plasmid

   #124849

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.13/AUG-FFLuc-3XFLAG

   Plasmid

   #127333

   Purpose

   Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   Firefly luciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CV40

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nanog-2A-mCherry

   Plasmid

   #59995

   Purpose

   Inserts mCherry immediately downstream of the Nanog coding region

   Depositor

   Rudolf Jaenisch

   Article

   Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2A-mCherry

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. SiC-V1

   Plasmid

   #133041

   Purpose

   SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   dTomato

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

   Plasmid

   #124862

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

    Plasmid

    #188377

    Purpose

    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-mas-GRK2RH-Nluc

    Plasmid

    #158605

    Purpose

    Plasmid expressing GRK2(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Nanog-2A-GFP

    Plasmid

    #59994

    Purpose

    Inserts GFP immediately downstream of the Nanog coding region

    Depositor

    Rudolf Jaenisch

    Article

    Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2A-GFP

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJRH623

    Plasmid

    #171633

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-non-targeting
    mNeon
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-a and U6

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)/AUG-nLuc-3XFLAG

    Plasmid

    #127299

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

    Plasmid

    #163027

    Purpose

    FLiCRE TF with fast kinetics for HEK293T expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    CD4-MKII-f-hLOV1-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT007

    Plasmid

    #171634

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASTattB-dALFANb(3mut)-mGreenLantern

    Plasmid

    #219662

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
    

    Expression

    Insect

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-HBB-nLuc

    Plasmid

    #175432

    Purpose

    Used to generate IVT RNA for translation extracts

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    HBB-nLuc
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV8-cPE-C

    Plasmid

    #183539

    Purpose

    Deliver a split compact Prime Editor in vivo

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U1A

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV hSyn grpLight1.2

    Plasmid

    #175174

    Purpose

    genetically encoded GRP sensor grpLight1.2

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    hSyn-driven grpLight1.2
    

    Use

    AAV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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