59,541 results

1. pMCL-HA-MAPKK1-WT

   Plasmid

   #40808

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

2. Alpha 5 integrin-GFP

   Plasmid

   #15238

   Depositor

   Rick Horwitz

   Article

   Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

   Insert

   alpha 5 integrin (ITGA5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-cIAP2/pRK5

   Plasmid

   #27973

   Depositor

   Xiaolu Yang

   Article

   Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

   Insert

   CIAP2 (BIRC3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSV human p85 alpha (HA tag)

   Plasmid

   #11499

   Depositor

   Ronald Kahn

   Article

   Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

   Insert

   p85 alpha (PIK3R1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBad-CA TAG126

   Plasmid

   #105836

   Purpose

   Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 126

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_Puro_Telo_v2

   Plasmid

   #195139

   Purpose

   ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Telomere (RTEL1 Human)
   

   Expression

   Mammalian

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-GFP-dynein-2(D1091–Q4307)

   Plasmid

   #64064

   Purpose

   Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

   Depositor

   Andrew Carter

   Article

   Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

   Insert

   Cytoplasmic dynein-2 (DYNC2H1 Human)
   

   Tags

   GFP, His tag, and ZZ tag

   Expression

   Insect

   Mutation

   Deleted amino acids 1-1090, extra C-terminal vali…

   Promoter

   Polyhedrin

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBOB-VE-cadherin-GFP

   Plasmid

   #118732

   Purpose

   To express VE-cadherin tagged with GFP on C-terminus

   Depositor

   John Cooper

   Article

   Kim et al Mol Biol Cell. 2018 Jul 15;29(13):1693-1

   Insert

   Cadherin 5 (CD144) (CDH5 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   I503T (Common SNP-see comments)

   Promoter

   CMV

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER20-hMYOD1-3xHA (#730)

   Plasmid

   #78328

   Purpose

   Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tag

   Depositor

   Matthew Alexander, Louis Kunkel

   Insert

   MYOD1 (MYOD1 Human)
   

   Use

   Lentiviral

   Tags

   3x HA tag

   Promoter

   TRE2

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-PRMT6

    Plasmid

    #164697

    Purpose

    Expression of PRMT6 with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

    Insert

    PRMT6 (PRMT6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIN4-EF2-O2S

    Plasmid

    #21162

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4, Sox2 (SOX2, POU5F1 Human)
    

    Use

    Lentiviral; Reprogramming

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_SMURF2_WT

    Plasmid

    #82259

    Purpose

    Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SMURF2 (SMURF2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Clathrin LC-15

    Plasmid

    #55019

    Purpose

    Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin LC (CLTB Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. p11L2w

    Plasmid

    #37331

    Depositor

    John Schiller

    Article

    Kieback et al Virology. 2006 Feb 5;345(1):199-208. Epu

    Insert

    HPV11 L2
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hsyn-GRAB_5-HT1.0

    Plasmid

    #140552

    Purpose

    Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    5-HT sensor GRAB_5-HT1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. TDP-43 mRuby

    Plasmid

    #216225

    Purpose

    TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

    Depositor

    Jennifer Lee

    Article

    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuidPuro-hTP53_sgRNA-1

    Plasmid

    #88853

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-puro HGF

    Plasmid

    #10901

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    HGF (HGF Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. 138-dCas9-Dnmt3a

    Plasmid

    #84570

    Purpose

    PiggyBac transposon system construct with dCas9-Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1 Utr230-EGFP-3XNLS

    Plasmid

    #58466

    Purpose

    Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Inserts

    Utr230 with 3X glycine linker (UTRN Human)
    3X NLS with 3X stop codons
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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