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pDONR223-ULK4

Plasmid

#23849

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ULK4 (ULK4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SRPK2

Plasmid

#23766

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SRPK2 (SRPK2 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKAA1

Plasmid

#23871

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAA1 (PRKAA1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLKO_HA.V5.FLAG_NGFR

Plasmid

#158250

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HA.V5.FLAG

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKG2

Plasmid

#23435

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKG2 (PRKG2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RPS6KC1

Plasmid

#23460

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KC1 (RPS6KC1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PAK2

Plasmid

#23655

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK2 (PAK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-LOC340371

Plasmid

#23545

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC340371 (NRBP2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcdna6.2- myc ULK1 k46I

Plasmid

#27630

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 K46I (Myc Mouse)

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

Plasmid

#159698

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ITGB1BP3

Plasmid

#23393

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ITGB1BP3 (NMRK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Anti-GABA(A)R, Alpha5 [N415/24R]

Plasmid

#149456

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha5 (Human). Derived from hybridoma N415/24.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Alpha5 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA5 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-NME1

Plasmid

#23573

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME1 (NME1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_MYC_WT

Plasmid

#82084

Purpose

Gateway Donor vector containing MYC , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MYC (MYC Human)

Use

Gateway entry vector

Mutation

L1M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIP5K1B

Plasmid

#23848

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K1B (PIP5K1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

Plasmid

#71829

Purpose

Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

U6

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-PANK4

Plasmid

#23419

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PANK4 (PANK4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NME4

Plasmid

#23584

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME4 (NME4 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMRX-IP-EGFP-PA-BAX(ER)

Plasmid

#228941

Purpose

Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
BAX (BAX Human)
Cyb5a (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus and C-terminus are deleted. BAX(3-171)…

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-UHMK1

Plasmid

#23433

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

UHMK1 (UHMK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only