We narrowed to 16,915 results for: EMB

1. Atg24 1B

   Plasmid

   #197046

   Purpose

   His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

   Insert

   Atg24
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBad-HisB-GRvT

   Plasmid

   #87363

   Purpose

   Expresses GRvT, a tandem heterodimer green and red fluorescent protein

   Depositor

   Robert Campbell

   Article

   Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

   Insert

   GRvT
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   AraBad

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-cdc2 (716)

   Plasmid

   #61840

   Purpose

   mammalian expression of cdc2

   Depositor

   Jonathon Pines

   Article

   Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

   Insert

   cdc2 (CDK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-ShhN-Ren

   Plasmid

   #37677

   Depositor

   Philip Beachy

   Article

   Ma et al Cell. 2002 Oct 4;111(1):63-75.

   Insert

   shh (Shh Mouse)
   

   Use

   Tags

   renilla luciferase

   Expression

   Mammalian

   Mutation

   soluble form (see comments)

   Promoter

   CMV

   Available since

   Nov. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pLP3

   Plasmid

   #209958

   Purpose

   Entry vector to store Level 0 broad-host range L. plantarum replication origin parts

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. C125-E01: stuffer

   Plasmid

   #162900

   Purpose

   Standard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectors

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   stuffer fragment for vector controls
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOGG026

   Plasmid

   #113992

   Purpose

   Level 1 golden gate cloning vector for low copy number stable environmental gene expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mTB3-Myc tag

   Plasmid

   #210205

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   Myc-tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS416-PHO5-GFP-Osh1

   Plasmid

   #58835

   Purpose

   Expresses GFP-Tagged Osh1 in yeast

   Depositor

   Tim Levine

   Article

   Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

   Insert

   Osh1 (SWH1, OSH1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. GR A458T

    Plasmid

    #133410

    Purpose

    overexpression of a mutant human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A458T

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. qTAG-C-moxGFP-Blast

    Plasmid

    #207710

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. saposin B^W

    Plasmid

    #161759

    Purpose

    Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

    Depositor

    Sam Gellman

    Article

    Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

    Insert

    saposin B R38_W
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    changed Arginine 38 to Tryptophan

    Promoter

    T7

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRc/CMV HA TrkA

    Plasmid

    #39977

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkA (Ntrk1 Rat)
    

    Use

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    endogenous signal sequence was replaced with BM40…

    Promoter

    CMV

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TagBFP2- PIP4K2CD280K

    Plasmid

    #202731

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:PIP4K2C (D280K) (PIP4K2C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. qTAG-C-3xHA-Zeo

    Plasmid

    #207740

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. qTAG-N-Zeo-3xHA

    Plasmid

    #207707

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEF1α-Cre-2A-miRFP670nano3

    Plasmid

    #207747

    Purpose

    Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-miRFP670nano3
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pTK81_GFP-4.1R

    Plasmid

    #46352

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1R (EPB41 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    pBABE

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACEBac1-GCP4

    Plasmid

    #178070

    Purpose

    Insect cell expression of GCP4

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    GCP4 (TUBGCP4 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-Gncp-iGluSnFR

    Plasmid

    #107337

    Purpose

    Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

    Insert

    GltI based and noncircularly permutated glutamate biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge