We narrowed to 10,611 results for: t7

1. pET28a-SpySrtA

   Plasmid

   #51139

   Purpose

   expression plasmid for N-terminal his tagged S.pyogenes SrtA

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.pyogenes SrtA
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-80

   Promoter

   T7

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIVT-dCas9-EGFP

   Plasmid

   #174442

   Purpose

   in vitro transcription template for dCas9-EGFP

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Cas9 Endonuclease Dead
   

   Use

   In vitro transcription template

   Tags

   EGFP

   Promoter

   T7 SP6

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMR-A-5

   Plasmid

   #138519

   Purpose

   P3c

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   T7-RNAP
   

   Use

   Synthetic Biology

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AviTag-SpyTag003-MBP

   Plasmid

   #184227

   Purpose

   Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   AviTag-SpyTag003-MBP
   

   Tags

   AviTag, His6, SpyTag003, and Thrombin cleavage si…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBSFI-myr-akt1

   Plasmid

   #49186

   Purpose

   cDNA of Akt1 with myristyolation signal in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Promoter

   T7

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLIC-EcTOP

   Plasmid

   #224463

   Purpose

   Expression of recombinant Escherichia coli topoisomerase I

   Depositor

   Yuk-Ching Tse-Dinh

   Article

   Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

   Insert

   Escherichia coli TopA
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. UbcH5B

   Plasmid

   #60443

   Purpose

   Express E2 UbcH5B in E. Coli

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   UbcH5B (UBE2D2 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pTrcHisB-TRF2

   Plasmid

   #50488

   Purpose

   E. Coli N-term HexHis Tagged Protein Expression Vector

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 (TERF2 Human)
   

   Tags

   6x His, T7 tag, and TEV protease site

   Expression

   Bacterial

   Mutation

   Contains amino acids 3-500

   Promoter

   pTrc

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 CIRBP

   Plasmid

   #67055

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CIRBP (CIRBP Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. hDHFR

    Plasmid

    #184707

    Purpose

    bacterial expression of human DHFR

    Depositor

    Peter Wright

    Article

    Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

    Insert

    DHFR (DHFR Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT138b-proB

    Plasmid

    #138515

    Purpose

    P3f

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMBP-SpyCatcher

    Plasmid

    #79053

    Purpose

    Expresses maltose binding protein (MBP) SpyCatcher fusion

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    MBP-SpyCatcher
    

    Tags

    6xHis and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Derived from pDEST14-SpyCatcher (Plasmid #35044)…

    Promoter

    T7

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Venus-StagRFP-AktAR

    Plasmid

    #173020

    Purpose

    Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Venus-StagRFP-AktAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-PACT

    Plasmid

    #50352

    Purpose

    bacterial expression of PACT

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    PACT (PRKRA Human)
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEXP1/Joseph2-Multitagged Plasmid

    Plasmid

    #226724

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pEXP1/Joseph2
    

    Tags

    6xHis, HA, Myc, GFP, Flag, V5

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EcNusGFL

    Plasmid

    #113122

    Purpose

    Expresses full-length E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG (nusG Escherichia coli)
    

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-28a-YTH domain of YTHDF3

    Plasmid

    #228777

    Purpose

    Protein expression plasmid for YTH domain of YTHDF3 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF3 (YTHDF3 Human)
    

    Tags

    6*His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET51b(+)_H-Luc

    Plasmid

    #113924

    Purpose

    Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET20b-NLuc-MBP-6xHis

    Plasmid

    #135931

    Purpose

    Expresses NanoLuciferase in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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