161,152 results

1. pEG BacMam

   Plasmid

   #160451

   Purpose

   vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330.puro

   Plasmid

   #110403

   Purpose

   CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti SYN-dCas9-KRAB-MeCP2

   Plasmid

   #155365

   Purpose

   Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

   Depositor

   Jeremy Day

   Article

   Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   3x FLAG and 7x His

   Expression

   Mammalian

   Mutation

   Promoter

   SYN

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBTet-SNAP-ALMS1

   Plasmid

   #136828

   Purpose

   Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-ALMS1 (ALMS1 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL_SRSF2_WT_mCherry

   Plasmid

   #84020

   Purpose

   expression of SRSF2 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   SRSF2 (SRSF2 Human)
   

   Use

   Lentiviral

   Tags

   2TA-mCherry and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-mitoGshF-blasticidin

   Plasmid

   #210314

   Purpose

   Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

   Expression

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-CD14

   Plasmid

   #13645

   Depositor

   Doug Golenbock

   Insert

   CD14 (CD14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

   Plasmid

   #230988

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Cre-dependent, membrane-localized optopatch for neuronal dendrites
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. FgH1tUTG

   Plasmid

   #70183

   Purpose

   Inducible expression of guide RNA with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   H1-Tet-sgrna cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSGNluc

    Plasmid

    #186705

    Purpose

    A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

    Depositor

    Maria Leptin, Baubak Bajoghli

    Article

    Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

    Inserts

    GFP
    luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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