We narrowed to 9,849 results for: EPO

1. pKrox24(MapERK)d1EGFP (KroxDs)

   Plasmid

   #214912

   Purpose

   for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   d1EGFP under the MapERK promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MapkERK promoter

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

   Plasmid

   #202059

   Purpose

   Inducible AAV activator

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
   

   Use

   AAV; Aav with reporter

   Tags

   Expression

   Mammalian

   Mutation

   ITRs intact

   Promoter

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

   Plasmid

   #229839

   Purpose

   A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

   Plasmid

   #85453

   Purpose

   Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-sgRNA-Backbone-UP-TdTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   deletion of AA123-365 in tdTomato (please see dep…

   Promoter

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Halo-p58

   Plasmid

   #166896

   Purpose

   mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   p58 (Lman1 Rat)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   L405Q, R419S- please see depositor comments

   Promoter

   CMV

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-GAL4-IL35-IRES-mCherry-PGK-BFP

   Plasmid

   #223210

   Purpose

   Lentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   IL35 (IL12A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

   Plasmid

   #212487

   Purpose

   Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP

   Plasmid

   #223208

   Purpose

   Lentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   PDL1 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-noSV40-humanCOL2A1enhancer

   Plasmid

   #173961

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Enhancer region from COL2A1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO HIS HSPH2

    Plasmid

    #19535

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 4 (HSPA4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    mutations A381T, T732A, and an insertion of "…

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-4F2-mCherry

    Plasmid

    #73421

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4F2 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-ITR-PytR(PyOtR)-mCherry

    Plasmid

    #204870

    Purpose

    Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

    Insert

    M. mazei pyrrolysyl tRNA mutant "PyOtR"
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

    Promoter

    U6

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTBL210

    Plasmid

    #126451

    Purpose

    Expresses Cas9-XTEN-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTwist-SARS-CoV-2 Spike-deltaC18 HK.3

    Plasmid

    #212572

    Purpose

    Encodes SARS-CoV-2 spike for HK.3 pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LSB-hsa-miR-29a-3p

    Plasmid

    #103391

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-29a-3p target (MIR29A Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPGK-T7/2-CD44v6 (-cyt)

    Plasmid

    #137817

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region, R296K (please see dep…

    Promoter

    PGK

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA

    Plasmid

    #218795

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    CMV-CBA-hGBA1-cm-hGBA1-hGHpA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-noSV40-humanEC1.45

    Plasmid

    #173946

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97308

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEMS2242

    Plasmid

    #111897

    Purpose

    ssAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple321 (TUBB3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple321 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDN-D2irC6kwh

    Plasmid

    #44725

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    mCherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LSB-hsa-miR-29a-5p

    Plasmid

    #103392

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-29a-5p target (MIR29A Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SIN40C.SFFV.ARID3a.IRES.GFP

    Plasmid

    #169300

    Purpose

    Constitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    ARID3A (ARID3A Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid

    #174666

    Purpose

    Doxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Arid3a-3xFLAG (Arid3a Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDN-T2dMFot

    Plasmid

    #44558

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

    Inserts

    PTETREG promoter
    rtetR-M2::FFF
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

    Promoter

    PTETREG

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97311

    Purpose

    MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb MMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX2T-TUBG1

    Plasmid

    #171967

    Purpose

    pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

    Depositor

    Maria Alvarado-Kristensson

    Article

    Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

    Insert

    TUBG1 (TUBG1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    R275H (Please see depositor comments)

    Promoter

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQE-80L 2xHis-LRRK2 Armadillo 1-159

    Plasmid

    #186016

    Purpose

    2xHis-LRRK2 Armadillo 1-159

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    See depositor's comments below.

    Promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LSB-hsa-miR-331-3p

    Plasmid

    #103444

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-331-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-331-3p target (MIR331 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEMS2274

    Plasmid

    #111900

    Purpose

    ssAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple344 (TUBB3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple344 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge