We narrowed to 16,540 results for: puromycin

1. pRExT2A-CT-PmSc

   Plasmid

   #232549

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mScarlet
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBMN-AS-YY1

   Plasmid

   #154943

   Purpose

   Knocking down of human YY1 through shRNA and amiRNA targeting YY1

   Depositor

   Claes Wadelius

   Article

   Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

   Insert

   shRNA and amiRNA against YY1 (YY1 Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. F40 tension sensor

   Plasmid

   #101252

   Purpose

   Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-F40-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

   Plasmid

   #184396

   Purpose

   Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Promoter

   Tet-On 3G

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Tet-myr-CSK-GFP

   Plasmid

   #83470

   Purpose

   Lentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   CSK (CSK Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   doxycycline-inducible promoter

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 1051 pBabe puroL Akt

   Plasmid

   #9011

   Depositor

   William Sellers

   Article

   DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

7. FL tension sensor module

   Plasmid

   #101170

   Purpose

   Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

   Depositor

   Carsten Grashoff

   Article

   Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

   Insert

   YPet(short)-FL-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

   Plasmid

   #162335

   Purpose

   Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pN006

   Plasmid

   #188470

   Purpose

   Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   AsCas12a
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBabe-FLAG-Neuroserpin

    Plasmid

    #58261

    Purpose

    Retroviral vector for expressing Neuroserpin in mammalian cells

    Depositor

    Joan Massague

    Article

    Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

    Insert

    Neuroserpin (SERPINI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pMXs-IP-EGFP-mAtg5

    Plasmid

    #38196

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid

    #178087

    Purpose

    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    mScarlet-I mTOR signaling indicator (TOSI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLYS1-EMRE/RNAi resistant

    Plasmid

    #50056

    Purpose

    Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    EMRE-RNAi resistant (SMDT1 Human)
    

    Expression

    Mammalian

    Mutation

    RNAi resistant, silent mutant

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid

    #83107

    Purpose

    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide

    Depositor

    Andrew Brooks

    Inserts

    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    

    Use

    Lentiviral

    Tags

    2A self cleaving peptide

    Expression

    Mammalian

    Mutation

    R24C and p64 isoform

    Promoter

    hPGK and hPGK (single transcript)

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. px459-Hira KO sgRNA

    Plasmid

    #186938

    Purpose

    Hira KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Hira KO sgRNA (Hira Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAPM-D4 miR30-SUV39h1 ts2

    Plasmid

    #115881

    Purpose

    SUV39h1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h1 shRNA (SUV39H1 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe-MITF

    Plasmid

    #64888

    Purpose

    Retroviral expression of murine MITF

    Depositor

    Ivana de la Serna

    Article

    de la Serna et al J Biol Chem. 2006 Jul 21;281(29):20233-4

    Insert

    MITF (Mitf Mouse)
    

    Use

    Retroviral

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJP-HBB-nLuc

    Plasmid

    #175431

    Purpose

    Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' HBB leader
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAPM-D4 miR30-SUV39h1 ts1

    Plasmid

    #115880

    Purpose

    SUV39h1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h1 shRNA (SUV39H1 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge