We narrowed to 58,550 results for: tra

1. pLKO.1-GST-EGFP-GPICEAM7

   Plasmid

   #213718

   Purpose

   For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an hPGK promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-GST-EGFP-GPICEAM7

   Plasmid

   #213723

   Purpose

   For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Tags

   V5-tag-GST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDonor_RB-TnV2_pJEx

   Plasmid

   #213908

   Purpose

   Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYea5.1_C99_sfGFP

   Plasmid

   #207802

   Purpose

   Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-C100-sfGFP-GAL4 fusion protein
   

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYea5.1_Notch3_sfGFP

   Plasmid

   #207864

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein
   

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Hy_pRnrS eGFP SV40

   Plasmid

   #195062

   Purpose

   Expresses eGFP mRNA from the Drosophila RnrS promoter

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   RnrS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Hy_pAna eGFP SV40

   Plasmid

   #195063

   Purpose

   Expresses eGFP mRNA from the Drosophila Ana promoter

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   Ana

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR4 AvrRpm1

   Plasmid

   #209185

   Purpose

   pENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   AvrRpm1
   

   Use

   Gateway-compatible entry vector

   Promoter

   none

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPI230

   Plasmid

   #207431

   Purpose

   Safe haven knock-in plasmid for the act5 locus for C-terminal mNeonGreen fusions

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   C-terminal mNeonGreen
   

   Use

   Unspecified

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPI231

    Plasmid

    #207432

    Purpose

    Safe haven knock-in plasmid for the act5 locus for N-terminal mNeonGreen fusions

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    N-terminal mNeonGreen
    

    Use

    Unspecified

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac1-bullfrog saxiphilin

    Plasmid

    #140595

    Purpose

    protein expression

    Depositor

    Dan Minor

    Article

    Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

    Insert

    saxiphilin bullfrog
    

    Tags

    GFP-His10

    Expression

    Insect

    Mutation

    Deletion of M20 - please see depositor comment

    Promoter

    polyhedrin promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKE011

    Plasmid

    #207912

    Purpose

    Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS018

    Plasmid

    #207911

    Purpose

    Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS017

    Plasmid

    #207909

    Purpose

    Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS020

    Plasmid

    #207915

    Purpose

    Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKE012

    Plasmid

    #207914

    Purpose

    Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKE010

    Plasmid

    #207910

    Purpose

    Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS019

    Plasmid

    #207913

    Purpose

    Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3/HF-CDC50B

    Plasmid

    #209224

    Purpose

    Mammalian expression of CDC50B

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    CDC50B (TMEM30B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

    Plasmid

    #193050

    Purpose

    Encodes guide RNA expression targeting PX740 landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    Guide RNA
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge