164,827 results

1. p3xFLAG CMV14 / BRAF-V600E

   Plasmid

   #131723

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   CMV

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHO-UAS-mScarlet-I

   Plasmid

   #183147

   Purpose

   Yeat two-hybrid fluorecent reporter for genomic integration

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Insert

   mScarlet-I
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

   Plasmid

   #215010

   Purpose

   Express a Mitophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   pSu9 (Presequence of Fo-ATPase subunit 9)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-SP-FRB (T2098L) -sfGFP-TM-V5

   Plasmid

   #186227

   Purpose

   mRNA synthesis of rapalog-induced cell adhesion molecule

   Depositor

   Yoshihiro Morishita

   Article

   Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

   Insert

   mRNA synthesis of rapalog-induced cell adhesion molecule
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   FRB (T2098L)

   Promoter

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-MCS1-P2A-MCS2 (Neo)

   Plasmid

   #89180

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACT-Lex

   Plasmid

   #183149

   Purpose

   Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Insert

   GAL4 AD
   

   Use

   Yeat two-hybrid

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sec61 beta pAcGFP-C1 (802)

   Plasmid

   #62008

   Purpose

   mammalian expression of GFP-Sec61 beta

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. NW9

   Plasmid

   #133442

   Purpose

   Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCR-GW

   Plasmid

   #209374

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by SCR promoter

   Depositor

   Lucia Strader

   Article

   Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWOL-GW

    Plasmid

    #209373

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by WOL promoter

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG::Gdnf

    Plasmid

    #78209

    Purpose

    piggybac vector for mouse Gdnf expression

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    Gdnf (Gdnf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pGEX6P1 p37

    Plasmid

    #169015

    Purpose

    Expresses GST-p37 (human, codon optimized for expression in bacteria)

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    p37 (UBXN2B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    sequence was codon optimized for expression in E.…

    Promoter

    tac

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-ALPK1

    Plasmid

    #23630

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. PB533-42B3mutAC2-sfGFP

    Plasmid

    #186777

    Purpose

    RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

    Insert

    42B3mutAC2-scFv
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-CTSL

    Plasmid

    #158444

    Purpose

    Gateway Cloning compatible entry vector for the human CTSL gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSL (CTSL Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

    Plasmid

    #62945

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CVB3 IRES

    Plasmid

    #189968

    Purpose

    Part 2 plasmid to introduce the CVB3 IRES into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    CVB3 IRES
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV40-EF1a-hBCL10-SO

    Plasmid

    #75256

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs, EF1a

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. ALPK1 gRNA (BRDN0001146195)

    Plasmid

    #76082

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ALPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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