We narrowed to 7,668 results for: CCH

1. pEK19

   Plasmid

   #210578

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Promoter

   BdBIP1pro

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSc-humanADAR2

   Plasmid

   #171106

   Purpose

   Expresses human ADAR2 in yeast.

   Depositor

   Brenda Bass

   Article

   Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

   Insert

   human ADAR2 (ADARB1 Human)
   

   Tags

   His12_TEV

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2796

   Plasmid

   #63641

   Purpose

   pCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEK39

   Plasmid

   #210599

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
   

   Tags

   mRuby3

   Expression

   Bacterial and Yeast

   Promoter

   SpH2Bpro

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJOG1368

   Plasmid

   #167301

   Purpose

   Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Insert

   FCY-UPP fusion gene
   

   Use

   Synthetic Biology; Moclo level 0 vector

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2793

   Plasmid

   #64043

   Purpose

   pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   TEF1 promoter and none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. NAX_CAGGS_CAS9_m4_KRAB_mcherry

   Plasmid

   #167878

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

8. PADH1-TIR1-9myc-URA3

   Plasmid

   #207016

   Purpose

   Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   TIR1
   

   Tags

   9xMyc

   Expression

   Yeast

   Promoter

   pADH1

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EC2_4_dCas9_VPR_sgRNA

   Plasmid

   #163709

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gal4DB-NS3-VP64-p65_IRES_mCherry

    Plasmid

    #112627

    Purpose

    Encodes the drug-activated transcription factor Gal4DB-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    Gal4DB-NS3-VP64-p65
    

    Tags

    AU1 (N term on NS3), HA (C term on NS3), and T7 (…

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHOHO2_Hsp82viiA

    Plasmid

    #61708

    Purpose

    Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.

    Depositor

    Dan Bolon

    Article

    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

    Insert

    Hsp82viiA (HSP82 Budding Yeast)
    

    Use

    Yeast integration vector targeting inserts to the…

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Heteromeric coiled coil inserted after amino acid…

    Promoter

    TEF1

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT004

    Plasmid

    #64759

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, V5 to an HA-6xHIS epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT001

    Plasmid

    #64758

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a V5 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHES830

    Plasmid

    #87944

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ADH1 (crippled)

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. EC2_3_dCas9_Mxi1_sgRNA

    Plasmid

    #163708

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    Mxi1+SV40 NLS
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Tags

    mRuby3

    Expression

    Yeast

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

    Plasmid

    #167902

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p403-BrdU-Inc

    Plasmid

    #71789

    Purpose

    BrdU-incorporating plasmid with selectable marker HIS3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Expression

    Yeast

    Promoter

    ADH1 and GPD

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSW149

    Plasmid

    #122248

    Purpose

    pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

    Depositor

    Sarah Walker

    Article

    Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

    Insert

    TIF4631 (TIF4631 Budding Yeast)
    

    Tags

    Intein-Chitin-binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKP3915

    Plasmid

    #113335

    Purpose

    Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Insert

    Artificial Transcription Factor (McIsaac et al, 2013)
    

    Expression

    Yeast

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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