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pLV hUbC-dCas9-MQ1(Q147L)-EGFP

Plasmid

#89793

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Publication

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR and Lentiviral

Tags

3*FLAG and T2A-EGFP

Expression

Mammalian

Mutation

Q147L, CAA-->CTA

Promoter

hUbC

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mNeonGreen-Rab7

Plasmid

#129603

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMT2 GATA1

Plasmid

#13626

Depositor

Anjana Rao

Publication

Monticelli et al J Biol Chem. 2004 Aug 27. 279(35):36210-

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
1257 pGEX4T3 GST FKHR

Plasmid

#10706

Depositor

William Sellers

Insert

FKHR (FOXO1 Human)

Tags

GST

Expression

Bacterial

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pEF-BFP

Plasmid

#138272

Purpose

Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

Depositor

Xiao Wang

Publication

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

BFP H66 PAM

Use

CRISPR

Expression

Mammalian

Mutation

H66, PAM motif

Promoter

Ef1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pWUR790

Plasmid

#101723

Purpose

Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

Depositor

John van der Oost

Publication

Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

PfAgo

Tags

Strep(II)-tag

Expression

Bacterial

Promoter

T7 Promotor

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HUW-TetO-hNanog

Plasmid

#25700

Depositor

Rudolf Jaenisch

Publication

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

Nanog (NANOG Human)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pDW363

Plasmid

#8842

Depositor

David Waugh

Publication

Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

Type

Empty backbone

Tags

MBP and biotin acceptor peptide

Expression

Bacterial

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Luc-pSUPER-Retro-GFP/Neo

Plasmid

#22133

Depositor

William Kaelin

Publication

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Publication

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Promoter

2xCLEAR

Available since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCXLE-Fbx15-cont2

Plasmid

#27081

Depositor

Shinya Yamanaka

Publication

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

FBXO15 (FBXO15 Human)

Expression

Mammalian

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-ZFN-AAVS1

Plasmid

#89707

Purpose

Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

Depositor

Dmitriy Mazurov

Publication

Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

Insert

ZFN-AAVS1

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Spectrin-M-cpstFRET

Plasmid

#61109

Purpose

Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

Depositor

Fred Sachs

Publication

Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

Insert

spectrin-cpstFRET

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-6xGLY-FLAG-kanMX6

Plasmid

#20751

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-Lyn-ICUE3

Plasmid

#61623

Purpose

Plasma membrane-targeted FRET biosensor for monitoring cAMP

Depositor

Jin Zhang

Publication

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

Lyn-ICUE3 (RAPGEF3 Human)

Tags

ECFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS2-FLAG-hEcto/Tif1g

Plasmid

#20902

Depositor

Stefano Piccolo

Publication

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal deletion of 37 aminoacids; Ectodermin …

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Publication

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDEST-eGFP-C1-VPS35

Plasmid

#163622

Purpose

human VPS35 cDNA C-terminally tagged with eGFP

Depositor

Josef Kittler

Publication

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

human VPS35 (VPS35 Human)

Tags

GFP

Expression

Mammalian

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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