4,183 results

1. pEF6.mCherry-TSG101

   Plasmid

   #38318

   Depositor

   Quan Lu

   Article

   Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   TSG101 (TSG101 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRII_TOPO_hNEAT1

   Plasmid

   #61518

   Purpose

   human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

   Depositor

   Archa Fox

   Article

   Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

   Insert

   NEAT1 (NEAT1 Human)
   

   Use

   General cloning vector

   Mutation

   *see comment below

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-TLR targeting vector

   Plasmid

   #64215

   Purpose

   Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Insert

   traffic light reporter (TLR) (AAVS1 Human)
   

   Use

   Human targeting

   Mutation

   see publication for details

   Promoter

   CAG

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

   Plasmid

   #103870

   Purpose

   High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ptet-RFP-shR-rtTA

   Plasmid

   #35625

   Purpose

   Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

   Depositor

   Howard Gu

   Article

   Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

   Type

   Empty backbone

   Use

   AAV and RNAi; Tet inducible

   Expression

   Mammalian

   Promoter

   Ptet

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. GPD-roGFP

   Plasmid

   #49436

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   GPD-roGFP2
   

   Use

   Adenoviral

   Tags

   Glycerol phosphate dehydrogenase, 5' to roGF…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRetrosuper Myc shRNA

   Plasmid

   #15662

   Depositor

   Martin Eilers

   Article

   Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

   Insert

   Myc shRNA (MYC Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. LeGO-C2

   Plasmid

   #27339

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-SHP2 WT

   Plasmid

   #8381

   Depositor

   Ben Neel

   Insert

   SHP2 (PTPN11 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSP3535

    Plasmid

    #46886

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Expression

    Bacterial

    Promoter

    PnisA

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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