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Showing: 5941 - 5960 of 66047 results

  3. pCW57.1 Lysosomal-METRIQ

    Plasmid
    #135401
    Purpose
    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity
    Depositor
    Eisuke Itakura
    Publication
    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10
    Insert
    DNAse II alpha (DNASE2 Human)
    Use
    Lentiviral
    Tags
    T2A, mCherry, and sfGFP
    Expression
    Mammalian
    Mutation
    Single-nucleotide polymorphism C432T
    Available since
    Feb. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135401-plasmid-map-sequence-id-273129

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  16. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86551-plasmid-map-sequence-id-171421

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  17. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84240
    Purpose
    Expresses GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84240-plasmid-map-sequence-id-160812

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  18. p5E-gfap

    Plasmid
    #75024
    Purpose
    Can be used to drive expression in zebrafish astrocytes.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    glial fibrillary acidic protein promoter (gfap Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    gfap
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75024-plasmid-map-sequence-id-450679

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  20. alpha catenin conformation sensor

    Plasmid
    #71709
    Purpose
    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-catenin FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71709-plasmid-map-sequence-id-142365

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Showing: 5941 - 5960 of 66047 results