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AKT3 gRNA (BRDN0001144935)

Plasmid

#76218

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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AKT3 gRNA (BRDN0001145071)

Plasmid

#76219

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT3 gRNA (BRDN0001147841)

Plasmid

#76220

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-2xFLAG-2xSTREP-CCND3-Puro

Plasmid

#172622

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD80-COMP5AP-AviTag-9xHis

Plasmid

#157189

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD80 (CD80 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pIG-709pUC19-tNGFR-P2A-IL10

Plasmid

#186117

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL10 (IL10 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDK4_WT

Plasmid

#82297

Purpose

Gateway Donor vector containing CDK4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDK4 (CDK4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pIGF1_P2 Promoter-FLuc

Plasmid

#154267

Purpose

Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

IGF-1 P2 Promoter (IGF1 Human)

Expression

Mammalian

Promoter

Human IGF-1 P2 Promoter

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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Kir6.2 K+ channel scFv [N363/71]

Plasmid

#190546

Purpose

Mammalian Expression of Kir6.2 K+ channel scFV. Derived from hybridoma N363/71.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj11 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-MEK5-pcw107

Plasmid

#64620

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

Flag and myristoylated

Promoter

PGK

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223_FTH1_WT

Plasmid

#81879

Purpose

Gateway Donor vector containing FTH1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FTH1 (FTH1 Human)

Use

Gateway entry vector

Mutation

K88E; L98Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Cavbeta1 Ca2+ channel [N7/18.1R]

Plasmid

#114497

Purpose

Mammalian Expression Plasmid of anti-Cavbeta1 Ca2+ channel (Human). Derived from hybridoma N7/18.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Cavbeta1 Ca2+ channel (Home sapiens) recombinant mouse monoclonal antibody (CACNB1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KozATG-dH6.2-2XG4S-actin

Plasmid

#73263

Purpose

Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)

Depositor

Marcel Bruchez

Article

Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

Insert

KozATG-dH6.2-2XG4S-actin (ACTB Synthetic, Human)

Tags

The FAP and actin are fused with 2 copies of a G4…

Expression

Mammalian

Mutation

The dH6.2 FAP has one cysteine in each domain cha…

Promoter

CMV

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-IDH1-P33S

Plasmid

#116409

Purpose

Lentiviral expression of IDH1 P33S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Mutation

P33S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATPIF1_WT

Plasmid

#81774

Purpose

Gateway Donor vector containing ATPIF1 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATPIF1 (ATP5IF1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ILDR2-COMP5AP-AviTag-9xHis

Plasmid

#157127

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ILDR2 (ILDR2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-R108G

Plasmid

#116291

Purpose

Lentiviral expression of EGFR R108G

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

R108G

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-S492R

Plasmid

#116303

Purpose

Lentiviral expression of EGFR S492R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

S492R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-P596L

Plasmid

#116284

Purpose

Lentiviral expression of EGFR P596L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

P596L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-E114K

Plasmid

#116238

Purpose

Lentiviral expression of EGFR E114K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

E114K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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