1. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pTKhc-yibDp-GFP-β20cp6

   Plasmid

   #127060

   Purpose

   Low phosphate inducible circular permutation of GFP, which is a substrate of the lon protease

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPcp6-beta20
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCKan-yibDp-CBD-hGLY

   Plasmid

   #134596

   Purpose

   Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   human glycine acyltransferase 2 (GLYATL2 )
   

   Use

   Tags

   chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSMART-Ala1

   Plasmid

   #65814

   Purpose

   NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   NADPH Alanine Dehydrogenase (ald Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   Insulated waaHp from E. coli

   Available since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV4Neo-murine IL-17RA-HA

   Plasmid

   #46859

   Purpose

   expresses mouse IL-17RA

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA (Il17ra Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pSMART-ala10

   Plasmid

   #87135

   Purpose

   NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Inserts

   NADPH Alanine Dehydrogenase (ald Synthetic)
   alanine exporter (alaE Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   waaHp from E. coli as part of operon with ald* an…

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. gh33

   Plasmid

   #106710

   Purpose

   expression of gRNA targeting B4GALNT3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   B4GALNT3 (B4GALNT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV4Neo-murine IL-17RASEFIR-HA

   Plasmid

   #46863

   Purpose

   expresses mouse IL-17RA with SEFIR deletion

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA-delta-SEFIR (Il17ra Mouse)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Deletion of SEFIR domain (AA 394-485)

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-HA-RAD52-WT

   Plasmid

   #235676

   Purpose

   Expresses HA-tagged wild type human RAD52 in mammalian cells.

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-HA-RAD52-∆C

   Plasmid

   #235677

   Purpose

   Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 302-410

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL20_mEGFP_ADIPOR2_FOXJ2

    Plasmid

    #205768

    Purpose

    Express mEGFP-tagged fusion protein, ADIPOR2_FOXJ2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ADIPOR2_FOXJ2 (ADIPOR2, FOXJ2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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