We narrowed to 3,114 results for: GAI

1. pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpA

   Plasmid

   #108752

   Purpose

   Expresses aa375 to C-terminus of Flp recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment aa375 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW2230_pCAG-PV1-GAI-L1-iCre-252C-NLS-BGHpA

   Plasmid

   #108726

   Purpose

   Expresses aa252 to C-terminus of Cre recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa252 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

   Plasmid

   #108833

   Purpose

   Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment aa28 to aa396
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS and GAI

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(Q61L)-GID1-mVenus-CAAX

   Plasmid

   #232262

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GAI1-92-KRas/N12 (KRAS Human)
   KRas/13C(Q61L)-GID1-mVenus-CAAX (KRAS Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(S17N)-GID1-mVenus-CAAX

   Plasmid

   #232263

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GAI1-92-KRas/N12 (KRAS Human)
   KRas/13C(S17N)-GID1-mVenus-CAAX (KRAS Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX

   Plasmid

   #232264

   Purpose

   Express split-Cdc42 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GID1-Cdc42/N12 (CDC42 Human)
   Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX (CDC42 Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   Cdc42-Q61L

   Promoter

   CMV

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(T17N)-GAI1-92-mVenus-CAAX

   Plasmid

   #232265

   Purpose

   Express split-Cdc42 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GID1-Cdc42/N12 (CDC42 Human)
   Cdc42/13C(T17N)-GAI1-92-mVenus-CAAX (CDC42 Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   Cdc42-T17N

   Promoter

   CMV

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

   Plasmid

   #214286

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

   Plasmid

   #214287

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84240

    Purpose

    Expresses GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84261

    Purpose

    Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    PYL1-VPR-p2a-GID1-ABI
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid

    #84257

    Purpose

    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid

    #106895

    Purpose

    Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9-ABI
    GID1-KRAB
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSC301

    Plasmid

    #31851

    Depositor

    Yossef Av-Gay

    Article

    Cowley et al Gene. 2001 Feb 21;264(2):225-31.

    Insert

    GFP (including ATG start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SOD

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pSC301b

    Plasmid

    #31852

    Depositor

    Yossef Av-Gay

    Article

    Cowley et al Gene. 2001 Feb 21;264(2):225-31.

    Insert

    GFP (including ATG start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SOD and none

    Available since

    Dec. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pSC300

    Plasmid

    #31820

    Depositor

    Yossef Av-Gay

    Article

    Cowley et al Gene. 2001 Feb 21;264(2):225-31.

    Insert

    GFP (lacking ATG start codon)
    

    Use

    Tags

    GFP (lacking ATG start codon)

    Expression

    Bacterial

    Mutation

    Promoter

    SOD

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pALACE

    Plasmid

    #31853

    Depositor

    Yossef Av-Gay

    Article

    Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

    Type

    Empty backbone

    Use

    Escherichia coli-mycobacterium shuttle plasmid

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Acetamidase operone

    Available since

    July 9, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pBS-UPRT

    Plasmid

    #69310

    Purpose

    expresses UPRT cDNA

    Depositor

    Chris Doe

    Article

    Gay et al Nat Protoc. 2014 Feb;9(2):410-20. doi: 1

    Insert

    UPRT cDNA
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pALOD4

    Plasmid

    #111026

    Purpose

    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A

    Promoter

    T7

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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