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We narrowed to 23,118 results for: Sis

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Showing: 81 - 120 of 23118 results

  7. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199221-plasmid-map-sequence-id-396541

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  12. pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

    Plasmid
    #72922
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Entry vector for gateway cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Promoter
    TRE tight
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72922-plasmid-map-sequence-id-138771

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  15. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199218-plasmid-map-sequence-id-396470

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  21. pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP

    Plasmid
    #168491
    Purpose
    Mammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
    Expression
    Mammalian
    Available Since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168491-plasmid-map-sequence-id-332602

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  27. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Thomas Norman
    Article
    Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224899-plasmid-map-sequence-id-443624

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  31. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

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  37. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

    Plasmid
    #205604
    Purpose
    Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion
    Depositor
    Tarun Kapoor
    Article
    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410
    Insert
    γ-tubulinΔGTP-RNAi-resistant-GFP
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    G2228A, C2231T, C2237T, G2240A, N229A
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205604-plasmid-map-sequence-id-405873

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  39. pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid
    #72924
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Entry vector for gateway cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Serine#37 changed to Alanine;Silent mutations mad…
    Promoter
    TRE tight
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72924-plasmid-map-sequence-id-138470

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  40. pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid
    #72617
    Purpose
    Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Lentiviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    Serine#37 changed to Alanine;Silent mutations mad…
    Promoter
    TRE tight
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72617-plasmid-map-sequence-id-138346

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Showing: 81 - 120 of 23118 results