We narrowed to 378 results for: pET-15b

1. pET15b-His-3C-irisin

   Plasmid

   #122612

   Purpose

   Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

   Depositor

   Harold Erickson

   Article

   Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

   Insert

   irisin (ectodomain of FNDC5) (Fndc5 Bovine, Rat, Mouse, Human)
   

   Use

   Tags

   Nt: histag-3C protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 polymerase

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. wt 6XHis-SNX9 pET15b

   Plasmid

   #34690

   Depositor

   Sandra Schmid

   Article

   Yarar et al Traffic. 2008 Jan;9(1):133-46. Epub 2007

   Insert

   Sorting nexin 9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET15b-17X-PTDH

   Plasmid

   #166786

   Purpose

   Phosphite dehydrogenase for NAD(P)H regeneration

   Depositor

   Wilfred van der Donk

   Article

   Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

   Insert

   Phosphite dehydroenase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A196R, T201S, A328T, E352N, and C356D- please see…

   Promoter

   T7

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b LFn-Fla

   Plasmid

   #84871

   Purpose

   Bacterial expression of LFn-Fla fusion

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Fla
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pET15b T7pCONS sfGFP

   Plasmid

   #154467

   Purpose

   pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15b LFn-Fla 3A

   Plasmid

   #84872

   Purpose

   Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Fla
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   3 C-terminal leucines have been replaced by alani…

   Promoter

   T7

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET15b-sfGO-Matryoshka-LE-LP

   Plasmid

   #100018

   Purpose

   Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   cpsfGFP-LSSmOrange
   

   Use

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Leu and Glu N-term linker and Leu and Pro C-term …

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15b I-SceI (Ctermhis -MH)

   Plasmid

   #195613

   Purpose

   Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Pet15b-Internalization Control

   Plasmid

   #186553

   Purpose

   Negative Control mediating endosomal localization

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   10R-8His-EGFP
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b-mLin28A-cHIS

    Plasmid

    #137998

    Purpose

    Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

    Depositor

    Judy Lieberman

    Article

    O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

    Insert

    mouse Lin28A, CSD and ZnFs (residues 36–186)
    

    Use

    Tags

    C-terminal His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b-eGO-Matryoshka-LE-LP

    Plasmid

    #100017

    Purpose

    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpEGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-15b-LanCL1

    Plasmid

    #154189

    Purpose

    To express LanCL1 in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 883 pET15b PTEN

    Plasmid

    #10936

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pET15b+PCNA1 P186C

    Plasmid

    #66173

    Purpose

    Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    P186C

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pET15b-His-IkappaBalpha(FL)

    Plasmid

    #44738

    Depositor

    Gourisankar Ghosh

    Insert

    IkappaBalpga (Nfkbia Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET15b-Fab1 (S. aureus)

    Plasmid

    #61691

    Purpose

    Inducible expression of the FabI enzyme from S. aureus

    Depositor

    Michael Johnson

    Insert

    FabI
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET15b-H74A-PvuRts1I

    Plasmid

    #86894

    Purpose

    express H74A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    W205A

    Promoter

    T7

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15b+PCNA2 L171C

    Plasmid

    #66177

    Purpose

    Expresses the L171C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b+PCNA1 G108C

    Plasmid

    #66140

    Purpose

    Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET15b-TGP-TwinStrep

    Plasmid

    #240251

    Purpose

    IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

    Depositor

    Krishna Reddy, Olga Boudker

    Article

    Reddy et al bioRxiv [Preprint]. 2024 Apr 25:2023.12.

    Type

    Empty backbone

    Use

    Tags

    10x HIS, Thrombin, Twin-Strep, and thermostable G…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b-ALFA-TwinStrep

    Plasmid

    #240252

    Purpose

    IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

    Depositor

    Krishna Reddy, Olga Boudker

    Article

    Reddy et al bioRxiv [Preprint]. 2024 Apr 25:2023.12.

    Type

    Empty backbone

    Use

    Tags

    10x HIS, ALFA, Thrombin, and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET15b-NHis6-NocL

    Plasmid

    #240398

    Purpose

    expresses N-His tagged NocL (fatty acid activating ligase) from Nodularia sp. 06071

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocL(fatty acyl-AMP ligase)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-15b-aδNb107

    Plasmid

    #220319

    Purpose

    Expresses the anti-IgD nanobody 107 (aδNb107) in the bacterial periplasm. aδNb107 binds to human IgD.

    Depositor

    James McDonnell

    Article

    Vester et al Sci Rep. 2025 Jul 8;15(1):24455. doi: 10

    Insert

    aδNb107
    

    Use

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-15b-aδNb367

    Plasmid

    #220320

    Purpose

    Expresses the anti-IgD nanobody 367 (aδNb367) in the bacterial periplasm. aδNb367 binds to human IgD.

    Depositor

    James McDonnell

    Article

    Vester et al Sci Rep. 2025 Jul 8;15(1):24455. doi: 10

    Insert

    aδNb367
    

    Use

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-15b-aδNb408

    Plasmid

    #220321

    Purpose

    Expresses the anti-IgD nanobody 408 (aδNb408) in the bacterial periplasm. aδNb408 binds to the Cδ2 domain of human IgD.

    Depositor

    James McDonnell

    Article

    Vester et al Sci Rep. 2025 Jul 8;15(1):24455. doi: 10

    Insert

    aδNb408
    

    Use

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-15b-aδNb571

    Plasmid

    #220322

    Purpose

    Expresses the anti-IgD nanobody 571 (aδNb571) in the bacterial periplasm. aδNb571 binds to human IgD.

    Depositor

    James McDonnell

    Article

    Vester et al Sci Rep. 2025 Jul 8;15(1):24455. doi: 10

    Insert

    aδNb571
    

    Use

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-15b-aδNb072

    Plasmid

    #204627

    Purpose

    Expresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.

    Depositor

    James McDonnell

    Article

    Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

    Insert

    aδNb072
    

    Use

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET15b (I-WcaINhis) K226I

    Plasmid

    #195620

    Purpose

    Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K226I mutation

    Depositor

    Frederick Gimble

    Insert

    I-WcaI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A1930T, A1931T

    Promoter

    T7

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET15b (I-WcaINhis) K125I

    Plasmid

    #195619

    Purpose

    Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K125I mutation

    Depositor

    Frederick Gimble

    Insert

    I-WcaI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A2030T, A2031T

    Promoter

    T7

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-15b(ETNPPL)

    Plasmid

    #192264

    Purpose

    Coding sequence of mouse ETNPPL is inserted in pET-15b vector

    Depositor

    Toshihide Yamashita

    Article

    Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

    Insert

    ethanolamine phosphate phospholyase (Etnppl Mouse)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b-GreBdm-D41N

    Plasmid

    #129691

    Purpose

    express E. coli GreB E82C/C68S/D41N

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GreB E82C C68S D41N (greB E. coli)
    

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET15b+C3(R112C/T180C)X

    Plasmid

    #108196

    Purpose

    Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    P450cam-PCNA3(R112C/T180C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET15b-E228A-PvuRts1I

    Plasmid

    #86896

    Purpose

    express E228A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E228A

    Promoter

    T7

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET15b-W215A-PvuRts1I

    Plasmid

    #86898

    Purpose

    express W215A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    W215A

    Promoter

    T7

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET15b-E71A-PvuRts1I

    Plasmid

    #86895

    Purpose

    express E71A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E71A

    Promoter

    T7

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET15b-D57A-PvuRts1I

    Plasmid

    #86892

    Purpose

    express D57A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D57A

    Promoter

    T7

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15b-R208A-PvuRts1I

    Plasmid

    #86891

    Purpose

    express R208A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R208A

    Promoter

    T7

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET15b-E20A-PvuRts1I

    Plasmid

    #86897

    Purpose

    express E20A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    Pvurts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E20A

    Promoter

    T7

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET15b-D70A-PvuRts1I

    Plasmid

    #86890

    Purpose

    express D70A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D70A

    Promoter

    T7

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge