Addgene

We narrowed to 139 results for: rab7;

Showing: 81 - 120 of 139 results

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mRFP-Rab5

Plasmid

#14437

Purpose

Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

Depositor

Ari Helenius

Article

Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

Insert

Rab 5 (RAB5A Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab5A Q79L

Plasmid

#28046

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Use

Tags

EGFP on N-term

Expression

Mammalian

Mutation

Q79L

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab5A S34N

Plasmid

#28045

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Use

Tags

EGFP on N-term

Expression

Mammalian

Mutation

S34N

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon deltaCT

Plasmid

#28040

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rubicon (RUBCN Human)

Use

Tags

3xFlag and EGFP

Expression

Mammalian

Mutation

delta880-end

Promoter

Available since

March 22, 2011

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon delta182

Plasmid

#28041

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rubicon (RUBCN Human)

Use

Tags

3xFlag and EGFP

Expression

Mammalian

Mutation

delta 1-182aa

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
Myc-Rab5A S34N

Plasmid

#28044

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

S34N

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-UbC-mCherry Rab5

Plasmid

#115239

Purpose

To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.

Depositor

Pantelis Tsoulfas

Insert

Rab5a (RAB5A Homo sapiens, Human)

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Human UbC

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-hTBK1-EGFP

Plasmid

#221553

Purpose

Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pZS218

Plasmid

#177641

Purpose

In vitro transcription of Patiria miniata Rab7

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Rab7

Use

In vitro transcription

Tags

mCherry

Expression

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

gRNA targeting human TBK1 (TBK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

u6

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE2-Bla(HA–RILP)

Plasmid

#102425

Purpose

HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Tet-responsive

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE2-Bla(HA–RILP-FLAG)

Plasmid

#102424

Purpose

Dual tagged RILP for expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Tet-Responsive

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-hTBK1-E696K-EGFP

Plasmid

#221554

Purpose

Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamate 696 to lysine

Promoter

EF1a

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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VPS29-YFP

Plasmid

#174520

Purpose

Expresses human VPS29 with a C-terminal YFP in mammalian cells

Depositor

Juan Bonifacino

Article

Rojas et al J Cell Biol. 2008 Nov 3;183(3):513-26. d

Insert

VPS29 (VPS29 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCold-TF-hRubicon

Plasmid

#64147

Purpose

For protein expression in E. coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Use

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pCold-TF-hPLEKHM1

Plasmid

#64146

Purpose

For protein expression in E.coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Mutation

Promoter

cspA

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pGAD-hPLEKHM1

Plasmid

#64145

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Tags

GAL4 activation domain

Expression

Yeast

Mutation

Promoter

ADH

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pGAD-hRubicon

Plasmid

#64144

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Use

Tags

GAL4 activation domain

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pBS L30 Ruby3-Rab6A

Plasmid

#135653

Purpose

Rab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab6A

Use

Tags

Ruby3

Expression

Mammalian

Mutation

Promoter

L30

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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35S:YFPc-RabG3b-DN

Plasmid

#102374

Purpose

fluorescent tagging. Plant expression of YFPc-RabG3b-DN

Depositor

Silke Robatzek

Insert

RabG3B (RABG3B Mustard Weed)

Use

Tags

YFPc

Expression

Plant

Mutation

T22N

Promoter

35S

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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35S:YFPc-RabG3b-WT

Plasmid

#102373

Purpose

fluorescent tagging. Plant expression of YFPc-RabG3b-WT

Depositor

Silke Robatzek

Insert

RabG3B (RABG3B Mustard Weed)

Use

Tags

YFPc

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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UBI10:RFP-RabG3B-DN

Plasmid

#102370

Purpose

fluorescent tagging. Plant expression of RFP-RabG3B-DN

Depositor

Silke Robatzek

Insert

RabG3B (RABG3B Mustard Weed)

Use

Tags

RFP

Expression

Plant

Mutation

T22N

Promoter

Ubi10

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
UBI10:RFP-RabG3B-WT

Plasmid

#102369

Purpose

fluorescent tagging. Plant expression of RFP-RabG3B-WT

Depositor

Silke Robatzek

Insert

RabG3B (RABG3B Mustard Weed)

Use

Tags

RFP

Expression

Plant

Mutation

Promoter

Ubi10

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
35S:YFPc-RabG3b-CA

Plasmid

#102372

Purpose

fluorescent tagging. Plant expression of YFPc-RabG3b-CA

Depositor

Silke Robatzek

Insert

RabG3B (RABG3B Mustard Weed)

Use

Tags

YFPc

Expression

Plant

Mutation

Q67L

Promoter

35S

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
UBI10:RFP-RabG3B-CA

Plasmid

#102371

Purpose

fluorescent tagging. Plant expression of RFP-35S:RabG3B-CA

Depositor

Silke Robatzek

Insert

RabG3B (RABG3B Mustard Weed)

Use

Tags

RFP

Expression

Plant

Mutation

Q67L

Promoter

Ubi10

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JB93

Plasmid

#128904

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab7AQL-BirA*-HA-MAO (RAB7A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JB94

Plasmid

#128905

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab7ATN-BirA*-HA-MAO (RAB7A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2_adRNA-MCP_ADAR1 DD_NES

Plasmid

#124630

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2_adRNA-MCP_ADAR2 DD_NES

Plasmid

#124706

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASP Flag Rab32 WT

Plasmid

#44833

Depositor

Matthew Scott

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

Flag

Expression

Insect

Mutation

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pUASP Flag Rab32 CA

Plasmid

#44834

Depositor

Matthew Scott

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

Flag

Expression

Insect

Mutation

Q79L (constitutive active)

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pUASP Flag Rab32 DN

Plasmid

#44835

Depositor

Matthew Scott

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

Flag

Expression

Insect

Mutation

T33N (dominant negative)

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pCMU-TLEr

Plasmid

#61187

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2_adRNA

Plasmid

#124624

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MS2_adRNA_RAB7A

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherryC1-Pacer

Plasmid

#216698

Purpose

Mammalian expression of Pacer

Depositor

James Hurley

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

Pacer (RUBCNL Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-80L 2xHis-Rab29

Plasmid

#186021

Purpose

2xHis-Rab29

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-Rab29 (RAB29 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCB306-GFP-Rab29

Plasmid

#198472

Purpose

lentiviral expression of GFP-Rab29

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab29 (RAB29 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab32

Plasmid

#31744

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab32 (Rab32 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Showing: 81 - 120 of 139 results

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