We narrowed to 9,389 results for: Coli
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Plasmid#51563PurposeExpression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertCRP/CAP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcPPK
Plasmid#38334DepositorInsertE. coli polyphosphate kinase (ppk Escherichia coli)
UseTags6xHIS, MBP, and TEVExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKM263-EcPPK
Plasmid#38333DepositorInsertE. coli polyphosphate kinase (ppk Escherichia coli)
UseTags6xHIS, GST, and TEVExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDM4-GLY
Plasmid#49808PurposeEncodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.DepositorInsertsphosphoglycerate kinase
glyceraldehyde-3-phosphate dehydrogenase
pyruvate dehydrogenase subunit E1
dihydrolipoamide acetyltransferase
dihydrolipoamide dehydrogenase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
ecALDH
Plasmid#67921PurposeExpresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coliDepositorInsertEscherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-sfGFP
Plasmid#51562PurposeExpression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)DepositorInsertsuperfolder GFP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CII
Plasmid#51566PurposeExpression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcII
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CI
Plasmid#51565PurposeExpression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcI
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLIC-EcTOP
Plasmid#224463PurposeExpression of recombinant Escherichia coli topoisomerase IDepositorInsertEscherichia coli TopA
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ec holE-pCDFDuet
Plasmid#241264PurposeOver-express E. coli Pol III subunit theta in E. coliDepositorInsertholE
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec dnaQ-pCDFDuet
Plasmid#241263PurposeOver-express E. coli Pol III subunit epsilon in E. coliDepositorInsertdnaQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec holA(Y316A)-FLAG-pET(11a)(ACH-)
Plasmid#238346Purposeover-expresses C-terminal FLAG tagged E. coli delta (Y316A) in E. coliDepositorInsertC-terminal FLAG tagged E. coli delta (Y316A) (holA E. coli)
UseTags3X FLAGExpressionBacterialMutationY316APromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJK640
Plasmid#72467PurposeEncodes Escherichia coli evgADepositorInsertresponse regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK641
Plasmid#72468PurposeEncodes Escherichia coli evgA (Plac noncoding)DepositorInsertresponse regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK646
Plasmid#72469PurposeEncodes Escherichia coli evgA (Plac coding)DepositorInsertresponse regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ec dnaE-pET(3c)
Plasmid#241262PurposeOver-express E. coli Pol III subunit alpha in E. coliDepositorInsertdnaE
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET11_DHNTPase
Plasmid#73154PurposeExpresses in E. coli, E. coli Dihydroneopterin triphosphate pyrophosphataseDepositorInsertORF17_DHNTPase_NUD
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN-pRSFDuet
Plasmid#238342Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta(wt) in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (wt) (dnaN E. coli)
UseTags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationnone (wt)PromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcA
Plasmid#107876PurposeExpresses E. coli RtcA in bacterial expression cellsDepositorInsertE. coli RtcA (rtcA Escherichia coli)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ec (gamma-only)-pET(11a)(ACH–)
Plasmid#241265PurposeOver-express E. coli Pol III subunit gamma-only in E. coliDepositorInsertdnaXZ (gamma-only)
UseTagsExpressionBacterialMutationNo expression of tauPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3E's)-pRSFDuet
Plasmid#238344Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
UseTags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96E; R103E; R105EPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3A's)-pRSFDuet
Plasmid#238343Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A) (dnaN E. coli)
UseTags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96A; R103A; R105APromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3S's)-pRSFDuet
Plasmid#238345Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96S; R103S; R105S) (dnaN E. coli)
UseTags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96S; R103S; R105SPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGU08
Plasmid#212149PurposePlasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.DepositorInsertcatalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK652
Plasmid#72470PurposeProduces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationMutated histidine-233 to glycinePromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
JBL6401
Plasmid#183838PurposesfGFP reporter under the control of WT E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
EcMscS
Plasmid#78555PurposeE. coli Mechanosensitive Channel of Small ConductanceDepositorInsertMechanosensitive Channel of Small Conductance
UseTags6x Histidine TagExpressionMutationPromoterT7 PromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-BBclone1
Plasmid#137069PurposeE. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238DepositorInsertsUseTagsExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…PromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB3033
Plasmid#113397PurposeTy3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducibleDepositorInsertTy3 Gag3 codon optimized for E. coli expression
UseTagsGag3-6XHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-NtC60αESNtLC60β
Plasmid#160878PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli.DepositorInsertrbcL, cpn60α, cpn60β, groES
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLNtC60αESC60β
Plasmid#160875PurposeExpression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coliDepositorInsertrbcL, cpn60α, cpn60β, groES
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBP-Bba_K822000
Plasmid#74094PurposeL-Lactate inducible promoter from E. coli MG1655DepositorInsertBba_K822000 (L-Lactate promoter - E. coli MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNAAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
JBL6423
Plasmid#183860PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Incumbent Rescue A regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6424
Plasmid#183861PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Mismatch Invader B regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6427
Plasmid#183864PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Mismatch Incumbent B regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6428
Plasmid#183865PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Incumbent Rescue B regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6410
Plasmid#183847PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch delta90-94 regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6411
Plasmid#183848PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch delta95-99 regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6412
Plasmid#183849PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch delta90-99 regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6413
Plasmid#183850PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Randomized A nt90-99 regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6415
Plasmid#183852PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Randomized A nt95-99 regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6416
Plasmid#183853PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Randomized B nt95-99 regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6417
Plasmid#183854PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Randomized A nt90-94 regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6418
Plasmid#183855PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Randomized B nt90-94 regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6419
Plasmid#183856PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Mismatch Invader A regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6420
Plasmid#183857PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Rescue Invader A regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6421
Plasmid#183858PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Full Rescue A regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6422
Plasmid#183859PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch Mismatch Incumbent A regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6402
Plasmid#183839PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch (G17C) regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6403
Plasmid#183840PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch (G37C) regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only