Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 25,897 results for: Nes

Clear keywords
Showing: 101 - 150 of 25897 results

  1. pEND-184_Cacnes_mCherry

    Plasmid
    #225610
    Purpose
    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225610-plasmid-map-sequence-id-451420

    Enlarge

  6. pEND-227_Cacnes_mCerulean

    Plasmid
    #225614
    Purpose
    Replicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    mCerulean
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225614-plasmid-map-sequence-id-451417

    Enlarge

  7. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

    Enlarge

  8. mCerulean-Farnesyl-5

    Plasmid
    #55367
    Purpose
    Localization: Membrane, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55367-plasmid-map-sequence-id-183116

    Enlarge

  10. mPlum-Farnesyl-5

    Plasmid
    #55968
    Purpose
    Localization: Plasma Membrane, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55968-plasmid-map-sequence-id-233116

    Enlarge

  12. pEND-321_Cacnes_ori

    Plasmid
    #225603
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225603-plasmid-map-sequence-id-451386

    Enlarge

  13. pEND-349_Cacnes_ori

    Plasmid
    #225604
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225604-plasmid-map-sequence-id-451376

    Enlarge

  19. iLOV-Farnesyl-5

    Plasmid
    #56516
    Purpose
    Localization: Membrane, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. Dendra2-Farnesyl-5

    Plasmid
    #57717
    Purpose
    Localization: Membrane, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. mCitrine-Farnesyl-5

    Plasmid
    #56558
    Purpose
    Localization: Membrane, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Tags
    FLAG and NES
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

    Enlarge

  24. pEND-164_Cacnes_pFAST

    Plasmid
    #225616
    Purpose
    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    pFAST
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225616-plasmid-map-sequence-id-451365

    Enlarge

  26. pLV V5-Nesprin-3

    Plasmid
    #175136
    Purpose
    Lentiviral expression of V5-tagged mouse Syne3
    Depositor
    Larry Gerace
    Insert
    Syne3 (Syne3 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175136-plasmid-map-sequence-id-352023

    Enlarge

  27. mApple-Farnesyl-5

    Plasmid
    #54899
    Purpose
    Localization: Plasma Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. mTurquoise-Nesprin3-C-18

    Plasmid
    #55572
    Purpose
    Localization: Nuclear Membrane , Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. pEND-224_Cacnes_sfGFP

    Plasmid
    #225611
    Purpose
    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225611-plasmid-map-sequence-id-451364

    Enlarge

  31. PS-CFP2-Farnesyl-5

    Plasmid
    #57227
    Purpose
    Localization: Membrane, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. mTagRFP-T2-Farnesyl-5

    Plasmid
    #58037
    Purpose
    Localization: Plasma Membrane, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  42. pEND-229_Cacnes_EforCP

    Plasmid
    #225615
    Purpose
    Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    EforCP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225615-plasmid-map-sequence-id-451383

    Enlarge

  43. pEND-303_Cacnes_tetR

    Plasmid
    #225618
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225618-plasmid-map-sequence-id-451367

    Enlarge

  44. pEND-304_Cacnes_tetR

    Plasmid
    #225619
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis and mCherry-V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225619-plasmid-map-sequence-id-451400

    Enlarge

  45. pEND-375_Cacnes_phlF

    Plasmid
    #225620
    Purpose
    C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    PhlF
    Use
    Synthetic Biology
    Tags
    6xHis and mCherry-V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225620-plasmid-map-sequence-id-451415

    Enlarge

  47. pEND-226_Cacnes_mCitrine

    Plasmid
    #225613
    Purpose
    Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    mCitrine
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225613-plasmid-map-sequence-id-451363

    Enlarge

Showing: 101 - 150 of 25897 results