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We narrowed to 23,988 results for: Nes

Showing: 101 - 150 of 23988 results

  1. pEND-184_Cacnes_mCherry

    Plasmid
    #225610
    Purpose
    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225610-plasmid-map-sequence-id-451420

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  4. pEND-193_Cacnes_HS

    Plasmid
    #225622
    Purpose
    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    HspR
    Use
    Synthetic Biology
    Tags
    pFAST-6XHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225622-plasmid-map-sequence-id-451414

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  5. pEND-229_Cacnes_EforCP

    Plasmid
    #225615
    Purpose
    Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    EforCP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225615-plasmid-map-sequence-id-451383

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  6. pEND-164_Cacnes_pFAST

    Plasmid
    #225616
    Purpose
    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    pFAST
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225616-plasmid-map-sequence-id-451365

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  7. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid
    #225617
    Purpose
    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225617-plasmid-map-sequence-id-451418

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  8. mEmerald-Kinesin11-N-18

    Plasmid
    #54137
    Purpose
    Localization: Motor Protein/Microtubule Filaments, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Kinesin11 (KIF11 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54137-plasmid-map-sequence-id-475409

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  9. pEND-303_Cacnes_tetR

    Plasmid
    #225618
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225618-plasmid-map-sequence-id-451367

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  10. pEND-304_Cacnes_tetR

    Plasmid
    #225619
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis and mCherry-V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225619-plasmid-map-sequence-id-451400

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  11. pEND-375_Cacnes_phlF

    Plasmid
    #225620
    Purpose
    C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    PhlF
    Use
    Synthetic Biology
    Tags
    6xHis and mCherry-V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225620-plasmid-map-sequence-id-451415

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  12. pEND-192_Cacnes_HS

    Plasmid
    #225621
    Purpose
    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    HspR
    Use
    Synthetic Biology
    Tags
    pFAST-6XHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225621-plasmid-map-sequence-id-451419

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  13. pEND-226_Cacnes_mCitrine

    Plasmid
    #225613
    Purpose
    Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    mCitrine
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225613-plasmid-map-sequence-id-451363

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  14. mEmerald-Farnesyl-5

    Plasmid
    #54093
    Purpose
    Localization: Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54093-plasmid-map-sequence-id-475392

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  26. tdTomato-Farnesyl-5

    Plasmid
    #58092
    Purpose
    Localization: Membrane, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    58092-plasmid-map-sequence-id-424771

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  28. mTagRFP-T2-Farnesyl-5

    Plasmid
    #58037
    Purpose
    Localization: Plasma Membrane, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pETSUMO2_Rhodoplanes-bGSDM

    Plasmid
    #212129
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Rhodoplanes bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212129-plasmid-map-sequence-id-419110

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  32. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214473-plasmid-map-sequence-id-425592

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  36. mPlum-Farnesyl-5

    Plasmid
    #55968
    Purpose
    Localization: Plasma Membrane, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55968-plasmid-map-sequence-id-233116

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  37. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

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  42. nes714tk/lacZ

    Plasmid
    #47614
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Article
    Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.
    Insert
    nestin 2nd intron fragment (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Expression
    Mutation
    714 bp from 3' end of 2nd intron
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. mCitrine-Farnesyl-5

    Plasmid
    #56558
    Purpose
    Localization: Membrane, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  44. mWasabi-Farnesyl-5

    Plasmid
    #56503
    Purpose
    Localization: Plasma Membrane, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. mEos3.2-Farnesyl-5

    Plasmid
    #57456
    Purpose
    Localization: Membrane, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57456-plasmid-map-sequence-id-393325

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  49. Dronpa-Farnesyl-5

    Plasmid
    #57272
    Purpose
    Localization: Membrane, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  50. miniSOG-Farnesyl-5

    Plasmid
    #62782
    Purpose
    Localization: Membrane, Excitation:448 / 473, Emission:500 / 528
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    miniSOG
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62782-plasmid-map-sequence-id-254842

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Showing: 101 - 150 of 23988 results