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Showing: 101 - 150 of 848 results

  1. cTRE-GFP-miR30_shPten.1523

    Plasmid
    #135667
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    shPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135667-plasmid-map-sequence-id-325441

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  3. pQdCas12a.sggfp(A)

    Plasmid
    #236038
    Purpose
    The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236038-plasmid-map-sequence-id-469933

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  4. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    SA-GFP reporter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  5. GBH-R-cmlc2GFP.1

    Plasmid
    #188004
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker cmlc2 GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; cmlc2 promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188004-plasmid-map-sequence-id-381042

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  6. pQdCas9-sggfp

    Plasmid
    #236184
    Purpose
    The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    Use
    Tags
    Expression
    Mutation
    Promoter
    Quorum sensing promoter
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    236184-plasmid-map-sequence-id-470109

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  7. GBH-R-insGFP.1

    Plasmid
    #188010
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188010-plasmid-map-sequence-id-381122

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  8. GBH-R-insGFP.2

    Plasmid
    #188011
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188011-plasmid-map-sequence-id-380751

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  9. GBH-R-insGFP.3

    Plasmid
    #188012
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188012-plasmid-map-sequence-id-380743

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  10. GBH-R-cmlc2GFP.3

    Plasmid
    #188006
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker cmlc2 GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; cmlc2 promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188006-plasmid-map-sequence-id-381114

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  11. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80811-plasmid-map-sequence-id-152800

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  12. AICSDP-49: MYL2-mEGFP

    Plasmid
    #114414
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114414-plasmid-map-sequence-id-220893

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  13. AICSDP-46: MYL7-mEGFP

    Plasmid
    #114413
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114413-plasmid-map-sequence-id-250980

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  14. KI(GAPDH)-P2A-EGFP-CMV-NeoR

    Plasmid
    #114009
    Purpose
    HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection
    Depositor
    Pavel Volchkov
    Article
    Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126
    Inserts
    GAPDH left arm (GAPDH Chicken)
    eGFP
    NeoR
    GAPDH right arm
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    -, CMV, and endogenous promoter (chicken GAPDH in…
    Available since
    Jan. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    114009-plasmid-map-sequence-id-261238

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  15. pQdCas12a.sggfp(B)

    Plasmid
    #236040
    Purpose
    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236040-plasmid-map-sequence-id-469924

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  16. pQdCas12a.sggfp(C)

    Plasmid
    #236042
    Purpose
    The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236042-plasmid-map-sequence-id-469925

    Enlarge

  17. GBH-R-gcryGFP.3

    Plasmid
    #188000
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188000-plasmid-map-sequence-id-381121

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  18. GBH-R-gcryGFP.1

    Plasmid
    #187998
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    187998-plasmid-map-sequence-id-381130

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  19. GBH-R-gcryGFP.2

    Plasmid
    #187999
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    187999-plasmid-map-sequence-id-380759

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  20. GBH-2aG4-insGFP.1

    Plasmid
    #188028
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188028-plasmid-map-sequence-id-381113

    Enlarge

  21. GBH-2aG4-insGFP.2

    Plasmid
    #188029
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188029-plasmid-map-sequence-id-381142

    Enlarge

  22. GBH-2aG4-cmlc2GFP.1

    Plasmid
    #188022
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; cmlc2 promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188022-plasmid-map-sequence-id-380745

    Enlarge

  23. GBH-2aG4-cmlc2GFP.2

    Plasmid
    #188023
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; cmlc2 promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188023-plasmid-map-sequence-id-381104

    Enlarge

  24. GBH-2aG4-cmlc2GFP.3

    Plasmid
    #188024
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; cmlc2 promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188024-plasmid-map-sequence-id-380895

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  27. mT2A-td-sfGFP-FNF

    Plasmid
    #182339
    Purpose
    Recombineering cassette plasmid for T2A-td-sfGFP-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-td-sfGFP-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182339-plasmid-map-sequence-id-358195

    Enlarge

  28. GBH-2aG4-gcryGFP.1

    Plasmid
    #188016
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188016-plasmid-map-sequence-id-381139

    Enlarge

  29. GBH-2aG4-gcryGFP.2

    Plasmid
    #188017
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188017-plasmid-map-sequence-id-381140

    Enlarge

  30. GBH-2aG4-gcryGFP.3

    Plasmid
    #188018
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188018-plasmid-map-sequence-id-381141

    Enlarge

  36. AICSDP-45: TTN-mEGFP

    Plasmid
    #114412
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114412-plasmid-map-sequence-id-220885

    Enlarge

  43. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

    Enlarge

  44. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid
    #135668
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgCR8
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135668-plasmid-map-sequence-id-325838

    Enlarge

  45. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid
    #135669
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135669-plasmid-map-sequence-id-325841

    Enlarge

  47. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid
    #236039
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236039-plasmid-map-sequence-id-469911

    Enlarge

  49. pQdCas9.luxR(mut)-sggfp

    Plasmid
    #236185
    Purpose
    The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    Quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    Use
    Tags
    Expression
    Mutation
    Promoter
    Quorum sensing promoter
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    236185-plasmid-map-sequence-id-471055

    Enlarge

Showing: 101 - 150 of 848 results