-
Plasmid#199799PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1333-DddA11'-C
Plasmid#199800PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_HD_G1333-DddA11'-N
Plasmid#199801PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1333-DddA11'-N
Plasmid#199802PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NI_G1333-DddA11'-N
Plasmid#199803PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NN_G1333-DddA11'-N
Plasmid#199804PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_HD_G1397-DddA11'-N
Plasmid#191611PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1397-DddA11'-N
Plasmid#191612PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NI_G1397-DddA11'-N
Plasmid#191613PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NN_G1397-DddA11'-N
Plasmid#191614PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_HD_G1397-DddA11'-C
Plasmid#191603PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11′), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NG_G1397-DddA11'-C
Plasmid#191604PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1397-DddA11'-C
Plasmid#191605PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1397-DddA11'-C
Plasmid#191606PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV0772
Plasmid#133516PurposeExpression of sfGFP tagged beta-tubulin to monitor microtubulesDepositorInsertpAde6(PmeI)-p(atb2)-sfGFP-atb2-terminator(atb2)-hphMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0607
Plasmid#133504PurposeExpression of mCherry targeted to the plasma membrane with an amphipathic helix of RitCDepositorInsertpAde6(PmeI)-p(act1)-mCherry-RitC-terminator(ScADH1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0763
Plasmid#133497PurposeExpression of nucleo-cytoplasmic mTagBFP2 in M-cellsDepositorInsertpAde6(PmeI)-p(mam1*)-mTagBFP2-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p181 pK7-HDAC1 (GFP)
Plasmid#11054DepositorInsertHDAC1 (Hdac1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
Spacer Switch Plasmid
Plasmid#215346PurposeFacilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.DepositorInsertOpaD spacer; Chloramphenicol Resistance Cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0783
Plasmid#133515PurposeExpression of mCherry tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0612
Plasmid#133505PurposeExpression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitCDepositorInsertpAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mHMGB4
Plasmid#31620DepositorInsertHMGB4 (Hmgb4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV0816
Plasmid#133510PurposeExpression of mTagBFP2 tagged CRIB domain to monitor active Cdc42DepositorInsertpAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTL
Plasmid#203176PurposeCentromeric yeast expression vector, leucine selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_18GZG
Plasmid#117228Purposeshble flanked by an endogenous GAPDH promoter and terminator systemDepositorInsertshble
UseThraustochytrid expressionTagsExpressionMutationPromoterGAPDHAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-neo
Plasmid#155026PurposeDinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) geneDepositorInsertamino 3'-glycosyl phosphotransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-pat
Plasmid#155028PurposeDinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, geneDepositorInsertPhosphinothricin N-acetyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-bsr
Plasmid#155027PurposeDinoflagelate backbone for blasticidin S-resistance gene (bsr)DepositorInsertBlasticidin-S deaminase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC:FCP:ShBle:FCP:EGFP
Plasmid#85987PurposeExpresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.DepositorInsertsShBle
EGFP
UseTagsExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
ss-cfSGFP2
Plasmid#37535DepositorInsertss-cfSGFP2 (cysteine-free SGFP2)
UseTagssignal sequence of α1-antitrypsin (M1-K34)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
aTY171_pPGK1_iRFP_WPRE
Plasmid#225351PurposeLentiviral vector for iRFP expressionDepositorInsertiRFP670
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
aTY173_pPGK1_mTagBFP2_WPRE
Plasmid#225352PurposeLentiviral vector for BFP expressionDepositorInsertBFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS-dar1-V5
Plasmid#45056DepositorInsertdar1 (dar1 Fly)
UseTagsV5ExpressionInsectMutationPromoterHS promoterAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 300
Plasmid#163115PurposeRed fluorescent protein-based glucose sensor for mammalian cells with higer affinityDepositorInsertRed Glifon 300
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 3000
Plasmid#163116PurposeRed fluorescent protein-based glucose sensor for mammalian cells with lower affinityDepositorInsertRed Glifon 3000
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p182 pK7-HDAC1 mut (GFP)
Plasmid#11055DepositorInsertHDAC1 (Hdac1 Mouse)
UseTagsGFPExpressionMammalianMutationDeletion of aa 1-56: deacetylase domain deletionPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6 Baf60b
Plasmid#24723DepositorInsertBaf60b (Smarcd2 Mouse)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pU6-STOP-shA1
Plasmid#14422DepositorInsertmodified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
UseCre/Lox and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
p180 pCIG-HDAC1 mut
Plasmid#11053DepositorInsertHDAC1 (Hdac1 Mouse)
UseTagsExpressionMammalianMutationDeletion of aa 1-56: deacetylase domain deletionPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dusp6DM_S159/197A
Plasmid#67684Purpose3' entry clone with activated Dusp6 (S159/167A)DepositorInsertdusp6 (dusp6 Zebrafish)
UseTagsExpressionMutationS159A, S197APromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Vkg::mCherry
Plasmid#163929PurposeExpression of GrabFP-ECM under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationG1767DPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pONSY
Plasmid#111873Purpose"Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).DepositorTypeEmpty backboneUseCapsaspora owczarzakiTagsExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Nrv1::tagBFP
Plasmid#163925PurposeExpression of GrabFP-B-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
UseCre/LoxTagstagBFPExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS-flt4um159-HA
Plasmid#67704PurposeExpression constructDepositorInsertflt4 (flt4 Zebrafish)
UseTagsHAExpressionMammalianMutationum159PromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::T48-Baz::vhhGFP4
Plasmid#163928PurposeExpression of GrabFP-A-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
Red Glifon Nega
Plasmid#163117PurposeRed fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cellsDepositorInsertRed Glifon Nega
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only