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Showing: 41 - 60 of 6125 results

  5. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

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  7. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

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  8. pD649-HAsp-KIT-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156885
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    KIT (KIT Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156885-plasmid-map-sequence-id-389764

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  11. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182682
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182682-plasmid-map-sequence-id-363082

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  12. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182685
    Purpose
    Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182685-plasmid-map-sequence-id-363019

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  13. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

    Plasmid
    #182686
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182686-plasmid-map-sequence-id-363076

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  14. pPD132.49

    Plasmid
    #1724
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only

  15. pPD138.11

    Plasmid
    #1721
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    EGFPN, EGFPC, IVSL, myo3-2341, myo3Tsa
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1721-plasmid-map-sequence-id-327

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  16. pPD157.63

    Plasmid
    #1710
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    vit2tsa, T20G511N, T20G511C, let8583', decoy
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1710-plasmid-map-sequence-id-316

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  17. pPD158.117

    Plasmid
    #1711
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc119tsa, RFPN, RFPivs2, RFPV105A, RFPivs4, RFPS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1711-plasmid-map-sequence-id-317

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  18. pPD107.09

    Plasmid
    #1703
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SupTrp5', SupTrp3', T7p, lacZN, OriF1&g…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1703-plasmid-map-sequence-id-309

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  19. pPD152.79

    Plasmid
    #1704
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    dpy30Tsa, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, …
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1704-plasmid-map-sequence-id-310

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  20. pPD187.24

    Plasmid
    #1723
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    pie1tsa, SPGFPN, SPGFPC, pie13', T3p, OriF1&…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1723-plasmid-map-sequence-id-329

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Showing: 41 - 60 of 6125 results