We narrowed to 2,171 results for: Xpa

1. CAG-loxP-3xpA-sfGFP-tdTomato T2A SGS XE

   Plasmid

   #113846

   Purpose

   Superfolder GFP and tdTomato linked by T2A (SGS linker)

   Depositor

   Mario Capecchi

   Insert

   sfGFP-T2A-tdTomato
   

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A APGST XE

   Plasmid

   #113848

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by T2A (APGST linker)

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-loxP-3xpA-sfGFP-tdTomato T2A APGST -XE

   Plasmid

   #113850

   Purpose

   Superfolder GFP and tdTomato linked by T2A (APGST linker, -XE)

   Depositor

   Mario Capecchi

   Insert

   sfGFP-T2A-tdTomato
   

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

   Plasmid

   #172479

   Purpose

   tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-loxP-3xpA-sfGFP-tdTomato long GSA linker E

   Plasmid

   #113855

   Purpose

   Superfolder GFP and tdTomato linked by long GSA linker

   Depositor

   Mario Capecchi

   Insert

   sfGFP-GSA_linker-tdTomato
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-loxP-3xpA-H2BsfGFP-tdTomato - long GSA linker E

   Plasmid

   #113845

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by long GSA linker

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-GSA_linker-tdTomato
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-loxP-3xpA-H2BsfGFP-tdTomato - opt GS PQR Var3 P2A -APGST E

   Plasmid

   #113847

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-P2A-tdTomato
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons

   Plasmid

   #172480

   Purpose

   H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

   Plasmid

   #67277

   Purpose

   inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

   Inserts

   first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
   second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
   

   Tags

   mTQ2 blue fluorescent and red flourescent gene ka…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2458

    Plasmid

    #36847

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    XPA-TALEN_Left (Xpa Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TAL2459

    Plasmid

    #36848

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    XPA-TALEN_Right (Xpa Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.Flag.HERC2-HECT-WT.6xHis

    Plasmid

    #39227

    Depositor

    Aziz Sancar

    Article

    Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

    Insert

    HERC2 HECT Domain (HERC2 Human)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.Flag.HERC2-HECT-C4762A.6xHis

    Plasmid

    #39228

    Depositor

    Aziz Sancar

    Article

    Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

    Insert

    HERC2 HECT Domain (HERC2 Human)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    C4762A

    Promoter

    T7

    Available Since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-MtAcKRS (human-opti)

    Plasmid

    #164195

    Purpose

    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MtAcKRS-478(human-opti)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    D76G; L325M; L329I; Y330F; L333A; C372F

    Promoter

    CMV

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-MfPylRS (human-opti)

    Plasmid

    #164081

    Purpose

    To express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MfPylRS(human-opti)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-MtPylRS (human-opti)

    Plasmid

    #164080

    Purpose

    To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MtPylRS-478(human-opti)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-MfBulKRS (human-opti)

    Plasmid

    #164196

    Purpose

    To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    Mf BulKRS(human-opti)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    Y269A;Y347F

    Promoter

    CMV

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAP14

    Plasmid

    #186262

    Purpose

    wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    wgaAB bicistronic
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Expression

    Bacterial

    Promoter

    LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid

    #223509

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Expression

    Mammalian

    Promoter

    CMV, SP6 and U6

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-AR Q48

    Plasmid

    #86428

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Poly-glutamine expansion, G130D

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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