We narrowed to 30,896 results for: ide

1. pAW13.lentiguide.mCherry

   Plasmid

   #104375

   Purpose

   mCherry vector for cloning in guides

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PP7 guide

   Plasmid

   #86168

   Purpose

   Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   PP7 guide
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   TRC

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuide-tagBFP

   Plasmid

   #217007

   Purpose

   lentiviral plasmid for gRNA cloning with selectable tagBFP expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-Crimson

   Plasmid

   #70683

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

   Depositor

   Daniel Bauer

   Article

   Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

   Inserts

   S. pyogenes sgRNA cassette
   Crimson Reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLHDR03_mCherry_tCD19_unique guide

   Plasmid

   #124062

   Purpose

   Construction of 1st donor for scarless genome editing with unique guide

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Inserts

   mCherry
   tCD19
   

   Use

   Mouse Targeting; Human targeting

   Promoter

   Human UbC modified

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOD1988-epiDEG

   Plasmid

   #89357

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Promoter

   Pdpy-7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide_CjCas9-Puro

   Plasmid

   #202564

   Purpose

   hU6-BsmBI-gRNA-Puro

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   CjCas9 sgRNA cassette
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiGuide-BlastR

   Plasmid

   #196970

   Purpose

   Lentiviral acceptor vector for pegRNAs with blasticidin resistance

   Depositor

   Leopold Parts

   Article

   Koeppel et al Nat Biotechnol. 2023 Feb 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   hU6

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hygro-iDEST

    Plasmid

    #75339

    Purpose

    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    TRE-DEST
    

    Expression

    Mammalian

    Promoter

    TRE-tight

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuide-RRBP1-1

    Plasmid

    #92156

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiGuide-BC-start

    Plasmid

    #127168

    Purpose

    Entry plasmid for two-step guide-barcode cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    N/A

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Puro-iDEST

    Plasmid

    #75336

    Purpose

    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    TRE-DEST
    

    Expression

    Mammalian

    Promoter

    TRE-tight

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-RRBP1-2

    Plasmid

    #92157

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiGuide-mMaroon

    Plasmid

    #217008

    Purpose

    lentiviral plasmid for gRNA cloning with selectable mMaroon expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiguide-puro_hORAI1sg

    Plasmid

    #174326

    Purpose

    gRNA targeting hORAI1 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETSUMO2_Bacteroidetes-bGSDM

    Plasmid

    #212124

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Bacteroidetes bGSDM
    

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNA-guideless

    Plasmid

    #129464

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Guideless gRNA backbone
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTurquoise-CIDEB-C-18

    Plasmid

    #55543

    Purpose

    Localization: , Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    CIDEB (CIDEB Human)
    

    Tags

    mTurquoise

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. lentiGuideFB-Puro-A

    Plasmid

    #192506

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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