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Plasmid#65524PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
P63-sp64 Poly A
Plasmid#59435Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 63
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
P29-sp64 Poly A
Plasmid#59434Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 29
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
P21-sp64 Poly A
Plasmid#59433Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 21
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
poly H35S SUMO1
Plasmid#221071PurposeExpression of poly H35S SUMO1 protein under T7 promoterDepositorInsertFive repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)
UseTagsHis and MBPExpressionBacterialMutationH35SPromotertacAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
poly E67R SUMO1
Plasmid#221072PurposeExpression of poly E67R SUMO1 protein under T7 promoterDepositorInsertFive repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)
UseTagsHis and MBPExpressionBacterialMutationE67RPromotertacAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
poly H35K SUMO1
Plasmid#221070PurposeExpression of poly H35K SUMO1 protein under T7 promoterDepositorInsertFive repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)
UseTagsHis and MBPExpressionBacterialMutationH35KPromotertacAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
poly H35R SUMO1
Plasmid#221069PurposeExpression of poly H35R SUMO1 protein under T7 promoterDepositorInsertFive repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)
UseTagsHis and MBPExpressionBacterialMutationH35RPromotertacAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcBAC3-POLY-pnRFP
Plasmid#201680PurposeBoost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)DepositorInsert813
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HO-Poly-KanMX4-HO
Plasmid#51662Purposeyeast plasmid for integration of target sequence at HO locus, Kan selectionDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Dnmt3b1-Poly(A)
Plasmid#65531PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locusDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Poly(A)‐NeoR
Plasmid#65526PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly-RGD
Plasmid#67002PurposeExpresses malE-poly-RGD in E. coliDepositorInsertmalE-poly-RGD
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Tet1-Poly(A)
Plasmid#65559PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Dnmt3b6-Poly(A)
Plasmid#65532PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locusDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 6PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Poly(A)‐NeoR
Plasmid#65528PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB‐mCh‐Poly(A)-PuroR
Plasmid#65529PurposePuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
DGP104- pSP64 Poly(A)700
Plasmid#70098Purposean RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kineticsDepositorInsert700bp insert from B. subtilis
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
DGP105- pSP64 Poly(A)900
Plasmid#70100Purposean RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kineticsDepositorInsert900bp insert from B. subtillis
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only