2,226 results

1. AAV-EF1a-jGCaMP8f-WPRE

   Plasmid

   #176756

   Purpose

   AAV-mediated expression of GCaMP8f under the EF1a promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nxn-1 beta Flag/pCMV

   Plasmid

   #197339

   Purpose

   Plasmid expressing an antigen targeting Neurexin 1beta

   Depositor

   Thomas Südhof

   Insert

   Neurexin 1beta (Nrxn1 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-No-HaloTag7-LC3

   Plasmid

   #184900

   Purpose

   Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. μ2-HA-D176A

   Plasmid

   #32754

   Depositor

   Alexander Sorkin

   Article

   Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

   Insert

   μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D176A

   Promoter

   CMV

   Available since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

5. AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

   Plasmid

   #107737

   Purpose

   In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

   Depositor

   Rylan Larsen

   Insert

   synaptophysin-GCaMP6s, mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-SEP-Glur2 (MCA10)

   Plasmid

   #64941

   Purpose

   mammalian expression of rat GluR2

   Depositor

   Jeremy Henley

   Article

   Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

   Insert

   GluR2 (Gria2 Rat)
   

   Use

   Tags

   SEP (superecliptic pHluorin)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CREB

   Plasmid

   #68550

   Purpose

   AAV expression of CREB

   Depositor

   Eric Nestler

   Article

   Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

   Insert

   CREB
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIGR AMPK WT

   Plasmid

   #27564

   Depositor

   Morris Birnbaum

   Insert

   AMP Acticated Protein Kinase (Prkaa2 Rat)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAd-PV-NLS-DsRed

   Plasmid

   #17287

   Depositor

   Anton Bennett

   Article

   Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

   Insert

   PV-DsRed-NLS-CD (Pvalb Rat)
   

   Use

   Adenoviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

    Plasmid

    #104496

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, and AAV1

    Insert

    jGCaMP7f
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-6p1-GST-ArcΔCTD

    Plasmid

    #119878

    Purpose

    Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of nt 832-1110

    Promoter

    tac

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA

    Plasmid

    #174900

    Purpose

    amber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Front Chem. 2021 Nov 11;9:768535. doi: 1

    Insert

    CRFR1 (Crhr1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A95TAG

    Promoter

    EF1

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-hSyn-Galpha-oA ONE-GO

    Plasmid

    #204335

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-oA ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pSAC5.02

    Plasmid

    #16077

    Depositor

    Lonny Levin, Jochen Buck

    Article

    Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

    Insert

    soluble adenylyl cyclase (Adcy10 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-ALK7 T194D

    Plasmid

    #80886

    Purpose

    mammalian expression of ALK7 T194D

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    ALK7 (Acvr1c Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T194D (constitutively active)

    Promoter

    CMV

    Available since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-Pak1 83-149

    Plasmid

    #12216

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Pak1 (Pak1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Dominant negative Pak1: autoinhibitory domain, aa…

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

    Plasmid

    #154781

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    CRFR1 (Crhr1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV5-rat ERK2-L73P/S151D

    Plasmid

    #40819

    Depositor

    Natalie Ahn

    Article

    Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

    Insert

    MAPK1 (Mapk1 Rat)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    L73P, S151D

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218866

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFsynW SYB2 reporter

    Plasmid

    #197276

    Purpose

    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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