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2,384 results

Showing: 581 - 600 of 2384 results

  1. pZac2.1-GfaABC1D-lck-jGCaMP8s

    Plasmid
    #176761
    Purpose
    AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Has service
    AAV1 and AAV9
    Insert
    lck-jGCaMP8s
    Use
    AAV
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    176761-plasmid-map-sequence-id-349053

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  3. pCAG-mEGFP-CaMKIIa (T286D)

    Plasmid
    #127391
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)
    Depositor
    Ryohei Yasuda
    Publication
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Aspartic acid
    Promoter
    pCAG
    Available since
    Jan. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    127391-plasmid-map-sequence-id-250431

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  7. LUC01 (L/Sec/P)

    Plasmid
    #112144
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Publication
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U)
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112144-plasmid-map-sequence-id-214867

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  8. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid
    #105715
    Purpose
    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.
    Depositor
    Rylan Larsen
    Has service
    AAV5
    Insert
    synaptophysin-GCaMP6s
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    105715-plasmid-map-sequence-id-207274

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  11. Cx26-msfGFP

    Plasmid
    #69016
    Purpose
    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Publication
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cx26 (Gjb2 Rat)
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69016-plasmid-map-sequence-id-124712

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  12. G protein alpha-s-HaloTag

    Plasmid
    #66993
    Purpose
    G protein alpha-s internally tagged with HaloTag and EE epitope
    Depositor
    Catherine Berlot
    Insert
    G-alpha-s-EE-HaloTag (Gnas Rat)
    Tags
    HaloTag flanked by SGGGGSGGGGS on each side was i…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66993-plasmid-map-sequence-id-122192

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  13. G protein alpha-s-CFP

    Plasmid
    #55793
    Purpose
    G protein alpha-s internally tagged with ECFP and EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-alpha-s-EE-ECFP (Gnas Rat)
    Tags
    ECFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    His was substituted for Asn164 in ECFP (Clontech)…
    Promoter
    CMV
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    55793-plasmid-map-sequence-id-86730

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  16. mCherry-CathespinB-6

    Plasmid
    #55007
    Purpose
    Localization: Lysosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CathespinB
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  20. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid
    #32522
    Depositor
    Mustafa Sahin
    Insert
    dominant negative Rheb (D60K) (Rheb Rat)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    D60K to make dominant negative
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 581 - 600 of 2384 results