2,339 results
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Plasmid#74309PurposeTricistronic expression of TRPV4, ferritin, and mCherry in mammalian cellsDepositorInsertTRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Rat, Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE
Plasmid#104492PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9InsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-YFP
Plasmid#55781PurposeThis G protein alpha-s construct contains internal insertions of YFP and the EE epitope.DepositorInsertalpha-s-EE YFP (Gnas Rat)
TagsEYFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationMet was substituted for Gln69 in EYFP (Clontech).…PromoterCMVAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK281 (mCherry2-Hygro)
Plasmid#72797PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Hygro
UseCRISPRTagsmCherry2ExpressionMammalianAvailable SinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1b-V5
Plasmid#162518PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.iGluSnFR3.v857.SGZ
Plasmid#178334PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV CAG iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV Cag Flex H2B Gcamp6f reverse
Plasmid#74155PurposeCalcium SensorDepositorInsertGcamp6f
UseAAVTagsH2BPromoterCAGAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt
Plasmid#175182PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn FLEX iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianPromoterhuman SynapsinAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1a-V5
Plasmid#162517PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
K44A dynamin 2 pEGFP
Plasmid#34687DepositorAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5 TGF beta type I receptor (T202D) Flag
Plasmid#14833DepositorInsertTGF beta type I receptor (Tgfbr1 Rat)
TagsflagExpressionMammalianMutationThreonine 202 mutated to aspartate. Constitutivel…Available SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc-mTOR (1750-2549 aa)
Plasmid#21747DepositorAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-E2419K
Plasmid#19994DepositorInsertmammalian target of rapamycin-E2419K (Mtor Rat)
TagsAU1ExpressionMammalianMutationChanged Glutamine 2419 to LysineAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE
Plasmid#104496PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, and AAV1InsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCAGAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
alpha1-EGFP
Plasmid#118952PurposeGABAA receptor expression (alpha1 subunit). EGFP tag at the C-terminus.DepositorAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG mEmerald-LAMP1
Plasmid#168513PurposeExpression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomesDepositorAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Arrb1-RFP
Plasmid#35401DepositorAvailable SinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
Tags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKH3-p190
Plasmid#15547PurposeExpression of HA-tagged p190 RhoGAPDepositorAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCer-4aa-VAMP2
Plasmid#53233PurposeFluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only