We narrowed to 1,214 results for: POLI

1. H2B-2A-GFP-IRESpuro2

   Plasmid

   #183746

   Purpose

   Expresses H2B-2A-GFP

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Use

   Tags

   GFP with 2A protease cleavage site between H2B an…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLMB577

   Plasmid

   #83778

   Purpose

   Phenylalanine biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   promoter of RL1860
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21a - CPX

   Plasmid

   #89620

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Cytosolic Tryparedoxin peroxidase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLMB686

   Plasmid

   #83780

   Purpose

   myo-Inositol biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   upstream region of RL4655 (intA)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCellFree_G03 PRKCA

   Plasmid

   #67115

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PRKCA Protein kinase C alpha type (PRKCA Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET21a - SodB

   Plasmid

   #89613

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Superoxide dismutase B
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLMB684

   Plasmid

   #83779

   Purpose

   Xylose biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   upstream region of RL2720 (rbsC)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCellFree_G03 PPARG

   Plasmid

   #67099

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PPARG (PPARG Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLMB687

   Plasmid

   #83781

   Purpose

   Sucrose biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   Region upstream pRL120556 containing all of pRL120555
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G03 PSMC1

    Plasmid

    #67087

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PSMC1 (PSMC1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    E275G, I287V and R294G

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLMB708

    Plasmid

    #83782

    Purpose

    Tartrate biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL0996 containing all of RL0997
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLMB710

    Plasmid

    #83783

    Purpose

    Formate biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL4393 (fdsG) containing all of RL4394
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLMB712

    Plasmid

    #83784

    Purpose

    Hesperetin biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLMB714

    Plasmid

    #83786

    Purpose

    C4-dicarboxylates biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL3424 (dctA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLMB705

    Plasmid

    #83787

    Purpose

    Mannitol biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL4218 (mtlB) containing all of RL4219
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLMB717

    Plasmid

    #83788

    Purpose

    Erythritol biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream pRL90085 containing all of pRL90087
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLMB737

    Plasmid

    #83790

    Purpose

    Malonate biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL0990 (matA) containing all of RL0989 (matR)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLMB738

    Plasmid

    #83791

    Purpose

    GABA biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL0102 (gabT) containing all of RL0103
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G03 TCF3

    Plasmid

    #67110

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCF3 (TCF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Truncated isoform

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pTU2-A-RFP

    Plasmid

    #72954

    Purpose

    Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCellFree_G03 YWHAB

    Plasmid

    #67063

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    YWHAB (YWHAB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pFE-dabAB2

    Plasmid

    #133002

    Purpose

    pFE carrying both the dabA2 (Uniprot: D0KWS7) and dabB2 genes (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a - NDH

    Plasmid

    #89626

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD(P)-dependent oxidoreductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBz106_CsoS2_MR

    Plasmid

    #140843

    Purpose

    His-tagged CsoS2 Middle Region (MR), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 Middle Region (MR)
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    only amino acids 257-603

    Promoter

    T7

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLz75_CB_operon_CsoS2_N3-N4

    Plasmid

    #140857

    Purpose

    Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-109 of CsoS2

    Promoter

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLz77_CB_operon_CsoS2_N3

    Plasmid

    #140859

    Purpose

    Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-108 and 220-235 of CsoS2

    Promoter

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLz78_CB_operon_CsoS2_delNTD

    Plasmid

    #140860

    Purpose

    Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-235 of CsoS2

    Promoter

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLz76_CB_operon_CsoS2_N4

    Plasmid

    #140858

    Purpose

    Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-189 of CsoS2

    Promoter

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBz109_CsoS2_NTD

    Plasmid

    #140842

    Purpose

    His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 N-terminal domain (NTD)
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 262-869

    Promoter

    T7

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBz110_CsoS2_CTD

    Plasmid

    #140844

    Purpose

    His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 C-terminal domain (CTD)
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-594

    Promoter

    T7

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge