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Addgene

We narrowed to 9,003 results for: Pol

Showing: 871 - 900 of 9003 results
  1. HIV-1-GFP ΔEnv CA M66I

    Plasmid
    #217440
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).
    Depositor
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    CA M66I
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. HIV-1-GFP ΔEnv CA Q67H/N74D

    Plasmid
    #217441
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).
    Depositor
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    CA Q67H,N74D
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. Sc FLAG-rad30-pRS403/GAL

    Plasmid
    #241260
    Purpose
    Over-express Sc FLAG-rad30 (Sc Pol eta) in yeast (integrated)
    Depositor
    Insert
    FLAG-rad30 (RAD30 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. Sc rev3-FLAG-pRS402/GAL

    Plasmid
    #241258
    Purpose
    Over-express Sc rev3-FLAG (Sc Pol zeta subunit) in yeast (integrated)
    Depositor
    Insert
    rev3-FLAG (REV3 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid
    #110426
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.
    Insert
    ELAVL1 HuR
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. Ec holD-pET(3c)

    Plasmid
    #237236
    Purpose
    over-express E. coli holD (Pol III psi subunit)
    Depositor
    Insert
    holD (psi) (holD E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. Ec dnaN-pET(11a)

    Plasmid
    #237238
    Purpose
    over-express E. coli dnaN (Pol III beta sliding clamp subunit)
    Depositor
    Insert
    dnaN (beta) (dnaN E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. Ec holA-pET(11a)(ACH-)

    Plasmid
    #237233
    Purpose
    over-express E. coli holA (Pol III delta subunit)
    Depositor
    Insert
    holA (delta) (holA E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. Ec holC-pET(3c)

    Plasmid
    #237235
    Purpose
    over-express E. coli holC (Pol III chi subunit)
    Depositor
    Insert
    holC (chi) (holC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. Ec dnaX(T-only)-pET(3c)

    Plasmid
    #237237
    Purpose
    over-express E. coli PolX (Pol III tau subunit - no gamma)
    Depositor
    Insert
    dnaX (tau only) (dnaX E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. Ec holB-pET(11a)(ACH-)

    Plasmid
    #237234
    Purpose
    over-express E. coli holB (Pol III delta prime subunit)
    Depositor
    Insert
    holB (delta prime) (holB E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Use
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pLKO.1-blast shKGA

    Plasmid
    #110420
    Purpose
    shKGA, silence glutaminase KGA isoform, blasticidin selection.
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pMLS256

    Plasmid
    #73715
    Purpose
    SapTrap destination vector for building combined sgRNA expression + repair template vectors
    Depositor
    Insert
    pU6::sgRNA
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    SapI insertion site
    Promoter
    C. elegans U6 snRNA pol III promoter
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  15. pRGEB32-BAR

    Plasmid
    #126072
    Purpose
    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 871 - 900 of 9003 results