We narrowed to 954 results for: kbp
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FRB-polySH35
Plasmid#175234PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRB
Plasmid#104475PurposePlasmid carrying FRB with C-terminal HA tag.DepositorInsertFRB (MTOR Human)
UsePhagemidTagsHA tagExpressionMutationincludes only the FRB domainPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld-GFP
Plasmid#53211PurposepEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld-GFP
UseTagsEGFP, Flag, and HAExpressionMutationPromoternoneAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
NCVB-ssGrl1-ESCargo*
Plasmid#140167PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophilaDepositorInsertssGrl1-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo
Plasmid#115420PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-C1-FKB-PI-PLCN187
Plasmid#139307PurposeN-terminal half of split PI-PLCDepositorInsertTagBFP2:FKBP1A(3-108):[GGSA]4GG:L. monocytogenes PI-PLC(1-187)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRBx2
Plasmid#104476PurposePlasmid carrying FRBx2 with C-terminal HA tag.DepositorInsertFRBx2 (MTOR Human)
UsePhagemidTagsHA tagExpressionMutationincludes only the FRB domainPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo*
Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo
Plasmid#115421PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-H11r1-2-DD-Cas9
Plasmid#85978PurposeExpresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cellsDepositorInsertDD-SpCas9
UseCRISPRTagsFKBP12 L106P DDExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo
Plasmid#140166PurposeExpresses a regulatable secretory cargo for Drosophila melanogasterDepositorInsertssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-FRB
Plasmid#191327PurposeExpresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FRB-dCer3-CAAX
Plasmid#191326PurposeExpresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-dCer-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-FRB-VMAc-CSarcin
Plasmid#70223PurposeExpresses VMA intein with C-terminal portion of alpha-sarcinDepositorInsertalpha Sarcin (C-terminal portion)
UseTagsFKBP-rapamycin binding (FRB) domain of the mammal…ExpressionMammalianMutationChanged Asp 28 to Cys, deleted amino acids 1-27PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FRB-VMAc-CGFP
Plasmid#70226PurposeExpresses C-terminal portion of GFP along with VMA intein for protein splicingDepositorInserteGFP
UseTagsFKBP-rapamycin binding (FRB) domain of the mammal…ExpressionMammalianMutationChanged Ile 129 to Cys, Deleted amino acids 1-128PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YF-RhoA(CA)
Plasmid#20153DepositorInsertYFP-FKBP-RhoA(CA) (RHOA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
YF-Rac1(CA)
Plasmid#20150DepositorInsertYFP-FKBP-Rac1(CA) (RAC1 Human)
UseTagsExpressionMammalianMutationconstitutively active mutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo*
Plasmid#140159PurposeExpresses a regulatable secretory cargo (without an ER export signal) for mammalian cellsDepositorInsertpiGH-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
YF-Rac1(DN)
Plasmid#20151DepositorInsertYFP-FKBP-Rac1(DN) (RAC1 Human)
UseTagsExpressionMammalianMutationdominant negative mutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJin-290
Plasmid#125718PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-CDepositorInsertPCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB-HA no-pA
Plasmid#82594Purpose3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGH51
Plasmid#107255PurposeHU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.DepositorInsertHU-FRB
UseSynthetic BiologyTagsFRBExpressionMutationPromoternative HU promoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only
