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Showing: 1451 - 1500 of 78335 results
  1. pAAV.hSynap-FLEX.SF-iGluSnFR.A184V

    Plasmid
    #106181
    Purpose
    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1
    Insert
    SF-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184V
    Promoter
    hSynapsin
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV.CAG.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106201
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

    Plasmid
    #106204
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184S + mRuby3 fusion
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106177
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    hSynapsin
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

    Plasmid
    #106205
    Purpose
    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184V + mRuby3 fusion
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

    Plasmid
    #106206
    Purpose
    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: S72A + mRuby3 fusion
    Promoter
    CAG
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV.CAG.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106202
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV.CAG.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106203
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    CAG
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pAAV.GFAP.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106195
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    GFAP
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106179
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    hSynapsin
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pAAV.GFAP.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106196
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    GFAP
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pAAV.GFAP.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106197
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    GFAP
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. lenti-sgRNA puro

    Plasmid
    #104990
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. lenti-sgRNA hygro

    Plasmid
    #104991
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. lenti-sgRNA blast

    Plasmid
    #104993
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106189
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    CAG
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  18. alpha actinin delta ABD-GFP

    Plasmid
    #66935
    Purpose
    Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted
    Insert
    Alpha-actinin 1 (ACTN1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    actin binding domain (amino acids 30–253) is dele…
    Promoter
    CMV
    Available since
    June 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  19. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106183
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    hSynapsin
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106190
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106185
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    hSynapsin
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  22. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106191
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    CAG
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  23. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106184
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    hSynapsin
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  24. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  25. pD649-HAsp-TNFRSF10B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156546
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Insert
    TNFRSF10B (TNFRSF10B Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  26. pUC19_GZG

    Plasmid
    #117226
    Purpose
    shble flanked by an endogenous GAPDH promoter and terminator system
    Depositor
    Insert
    shble
    Use
    Thraustochytrid expression
    Tags
    Expression
    Mutation
    Promoter
    GAPDH
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  27. FRP322

    Plasmid
    #159303
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized CitrineA206K under KanMX selection
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    V2del, S3del, F64L, A206K, L231H
    Promoter
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  28. pUCP20 PilB

    Plasmid
    #162845
    Purpose
    Expression of PilB with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pUCP20 PilBF120*

    Plasmid
    #162852
    Purpose
    Expression of PilB F120 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    F120Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pUCP20 PilBT125*

    Plasmid
    #162853
    Purpose
    Expression of PilB T125 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    T125Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  31. pUCP20 PilBN189*

    Plasmid
    #162854
    Purpose
    Expression of PilB N189 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    N189Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  32. pUCP20 PilBL193*

    Plasmid
    #162855
    Purpose
    Expression of PilB L193 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    L193Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  33. pUCP20 PilBK197*

    Plasmid
    #162856
    Purpose
    Expression of PilB K197 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    K197Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  34. pUCP20 PilBQ231*

    Plasmid
    #162857
    Purpose
    Expression of PilB Q231 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    Q231Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  35. pUCP20 PilBL240*

    Plasmid
    #162858
    Purpose
    Expression of PilB L240 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    L240Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  36. pUCP20 PilBS249*

    Plasmid
    #162859
    Purpose
    Expression of PilB S249 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    S249Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  37. pUCP20 PilBE309*

    Plasmid
    #162861
    Purpose
    Expression of PilB E309 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    E309Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  38. pUCP20 PilBF486*

    Plasmid
    #162862
    Purpose
    Expression of PilB F486 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    F486Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  39. pUCP20 PilBA516*

    Plasmid
    #162863
    Purpose
    Expression of PilB A516 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    A516Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  40. pUCP20 PilBE529*

    Plasmid
    #162864
    Purpose
    Expression of PilB E529 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    E529Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  41. pUCP20 PilBE532*

    Plasmid
    #162865
    Purpose
    Expression of PilB E532 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    E532Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  42. pUCP20 PilBK536*

    Plasmid
    #162866
    Purpose
    Expression of PilB K536 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    K536Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  43. pUCP20 PilBL37*

    Plasmid
    #162846
    Purpose
    Expression of PilB L37 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    L37Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  44. pUCP20 PilBS76*

    Plasmid
    #162847
    Purpose
    Expression of PilB S76 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    S76Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  45. pUCP20 PilBQ113*

    Plasmid
    #162848
    Purpose
    Expression of PilB Q113 Amber with a C-terminal histag
    Depositor
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    Q113Amber
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1451 - 1500 of 78335 results