Addgene

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cACTB

Plasmid

#236544

Purpose

For expression of ACTB in mammalian cells

Depositor

Sandra Citi

Article

Mauperin et al Nat Commun. 2025 Mar 13;16(1):2514. doi:

Insert

ACTB

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

hpACTB

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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cACTG1

Plasmid

#236543

Purpose

For expression of ACTG1 in mammalian cells

Depositor

Sandra Citi

Article

Mauperin et al Nat Commun. 2025 Mar 13;16(1):2514. doi:

Insert

ACTG1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

hpACTB

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep-D3:Luc

Plasmid

#192990

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D3

Promoter

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep-D1:Luc

Plasmid

#192988

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D1

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep-D2:Luc

Plasmid

#192989

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D2

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HyWnt3-ΔRep-D4:Luc

Plasmid

#192991

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D4

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A K31A

Plasmid

#105696

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 and 31 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-WW-CR-d-ZZ

Plasmid

#18071

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-CR-d-ZZ

Plasmid

#18075

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-EF1+EF2

Plasmid

#18080

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pet-ECHB(35-475)

Plasmid

#96890

Purpose

expresses His-tagged mouse ECHB (35-475) in E.coli cells

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

deleted amino acid 1-34

Promoter

T7

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZc-ScAct1*-thymosinB-8His

Plasmid

#111148

Purpose

Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

Act1 (ACT1 Budding Yeast)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

Promoter

AOX1

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING V155M

Plasmid

#102599

Purpose

Express STING V155M for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V155M

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING HAQ

Plasmid

#102600

Purpose

Express STING HAQ for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING HAQ+V155M

Plasmid

#102601

Purpose

Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V155M R71H-G230A-R293Q

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad2

Plasmid

#89827

Purpose

Knockdown human Smad2 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD2 shRNA (SMAD2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Use

Tags

TagBFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EGFP-IRES-Neo

Plasmid

#128660

Purpose

mammalian expression of EGFP with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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XE248 pCDNA3.1+zeo-VSV-KLHL12

Plasmid

#16761

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

pCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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