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pKLBac-Bat-FluA-trimer
Plasmid#229858PurposeInfluenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)DepositorInsertsPA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1a ActBT2ATetP2AP - H
Plasmid#229972PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and HygroDepositorInsertActBT2ATetP2AP - H
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1a ActBT2AmcherryP2AP -TagBFPH
Plasmid#229971PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-HygroDepositorInsertActBT2AmcherryP2AP -TagBFPH
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2
Plasmid#228829PurposeInsect cell expression of BICD2DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_HelperΔgIII
Plasmid#190433PurposeM13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.DepositorInsertM13 genes I-V and VII-XI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT033
Plasmid#225179PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (GmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1C2CMUNC2C
Plasmid#218674PurposeInsect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2CDepositorInsertMUNC13 (Unc13a Rat)
UseTagsGSTExpressionInsectMutationAmino acids1407-1453 deletedPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1177
Plasmid#217961PurposeDual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.DepositorInsertHistone H3 (HIST2H3PS2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBMeCP2G.10 (pc1577)
Plasmid#211728PurposeExpresses MeCP2 (aa1-76) GFP tagged in insect cellsDepositorInsertMeCP2 aa1-76
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.6G (pc2396)
Plasmid#211724PurposeExpresses MBD2 (aa153-235) GFP tagged in insect cellsDepositorInsertMBD2.6 aa153-235
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2Y.3 (pc1575)
Plasmid#211717PurposeExpresses MeCP2 (aa1-163) YFP tagged in insect cellsDepositorInsertMeCP2 aa1-163
UseTagsYFPExpressionInsectMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2mR (pc2519)
Plasmid#211716PurposeExpresses fl. MBD2 RFP tagged in insect cellsDepositorInsertMBD2
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBMBD4-C-GFP (pc1567)
Plasmid#211714PurposeExpresses fl. MBD4 GFP tagged in insect cellsDepositorInsertMBD4
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBMBD3-C-GFP (pc1566)
Plasmid#211713PurposeExpresses fl. MBD3 GFP tagged in insect cellsDepositorInsertMBD3
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBMBD1a-C-GFP (pc1564)
Plasmid#211711PurposeExpresses fl. MBD1 GFP tagged in insect cellsDepositorInsertMBD1
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHFdist_rm
Plasmid#162318PurposeP. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removedDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHF distal site is removedPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_69.11
Plasmid#194214PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN_TT mCh-V5-NES-EGFP-mCBP80
Plasmid#192313PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80DepositorInsertmCh-V5-NES-EGFP-mCBP80
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-IBB
Plasmid#192314PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-IBBDepositorInsertmCh-V5-NES-EGFP-IBB
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only
