We narrowed to 29,613 results for: des.2

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sgMST1/2-1

Plasmid

#229429

Purpose

knockout of MST1 and MST2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMST1/2-1 (MST1, STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-2-GAPDH

Plasmid

#83910

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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sgYAP-TAZ-2

Plasmid

#229452

Purpose

knockout of YAP1 and WWTR1 (TAZ)

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-TAZ-2 (YAP1, WWTR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pNICBio2_WDR5:2-334

Plasmid

#211027

Purpose

bacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR5:A2-C334 (WDR5 Human)

Tags

MHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHE

Expression

Bacterial

Mutation

wild type

Promoter

T7

Available Since

April 4, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLIII CKBs CRISPR with guides at 1-2 and 2-3

Plasmid

#238525

Purpose

Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha3

Depositor

Michael Hothorn

Insert

AtCK2alpha3 (AT2G23080 Mustard Weed)

Use

CRISPR

Expression

Plant

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

Plasmid

#184533

Purpose

Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-BA.2 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-BA.2-AVI

Plasmid

#184532

Purpose

Expresses SARS-CoV-2 NTD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-BA.2 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24-, P25-, P26-, A27S, G142D, V213G

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-BA.2-AVI

Plasmid

#184531

Purpose

Expresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-BA.2 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24-, P25-, P26-, A27S, G142D, V213G, G339D…

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir129-2-2

Plasmid

#46728

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir129-2-2 (MIR129-2 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

Plasmid

#223273

Purpose

Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

Plasmid

#212487

Purpose

Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 AY.2

Plasmid

#212434

Purpose

Encodes SARS-CoV-2 spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)

Plasmid

#22131

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo/pTER shEGFP#2 (w22-2)

Plasmid

#17480

Purpose

3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #2

Use

Lentiviral and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

Plasmid

#61787

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR with miR-200c MRE-2 mutated

Use

Luciferase

Mutation

miR-200c MRE-2 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167848

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis and AviTag

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Article

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-Dynamin 2 K44A

Plasmid

#22301

Depositor

Pietro De Camilli

Article

Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

Insert

Dynamin 2 K44A (Dnm2 Rat)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only