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EF1a_ETV2_P2A_Hygro_Barcode

Plasmid

#120436

Purpose

Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpa

Plasmid

#79551

Purpose

Expression of mouse Cebpa

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp alpha (Cebpa Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

Plasmid

#170322

Purpose

Expresses FLAG and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-rTetO-mCherry-T2A-RPL22-1xV5

Plasmid

#170325

Purpose

Tetracycline-inducible expression of V5 and mCherry tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

Plasmid

#170328

Purpose

Double-floxed inverse Orientation V5 and mCherry tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Nkx2.5

Plasmid

#72689

Purpose

Doxycyline regulated expression of Nkx2.5 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Nkx 2.5 (Nkx2-5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp2

Plasmid

#72693

Purpose

Doxycyline regulated expression of Mesp2 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 2 (Mesp2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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1068 pGL3 FasL promoter (no SV40 prom)

Plasmid

#9029

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Removed the SV40 promoter from pGL3

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
p2702-AGER-gRNA1

Plasmid

#216471

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2703-AGER-gRNA2

Plasmid

#216472

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2704-AGER-gRNA3

Plasmid

#216473

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

Plasmid

#216477

Purpose

lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

dsRed
tagBFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

Plasmid

#216478

Purpose

lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
tagBFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-EBF1

Plasmid

#96965

Purpose

Dox-inducible expression of Ebf1 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

Ebf1 (Ebf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NICD

Plasmid

#61540

Purpose

Expresses NOTCH-ICD in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

NOTCH-ICD (NOTCH1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NOTCH intracellular domain (aa1761-2555); A1943S…

Promoter

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pTRE3G-GSX2

Plasmid

#96964

Purpose

Dox-inducible expression of Gsx2 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 (Gsx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Isl1

Plasmid

#72696

Purpose

Doxycyline regulated expression of Isl1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Islet1 (Isl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_SRF

Plasmid

#72695

Purpose

Doxycyline regulated expression of SRF in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

serum response factor (SRF Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Irx4

Plasmid

#72692

Purpose

Doxycyline regulated expression of Irx4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Irx 4 (IRX4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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