-
Plasmid#208625Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 72~197, other residues not includedPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pxyl-rsaA
Plasmid#207250PurposeIntegrative plasmid for expressing rsaA gene (incl. its promoter) in Caulobacter crescentus at native Pxyl promoter regionDepositorInsertrsaA (promoter to codon sequence)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ARL16-His6
Plasmid#216484PurposeUsed for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tagDepositorInsertADP-ribosylation factor like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-msEGFP1.8
Plasmid#208619PurposeMammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.DepositorInsertmsEGFP1.8
UseTagsExpressionMammalianMutation(compared with pEGFP-C1, protein level) S30R, Y39…PromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHM1_ARATH
Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-blast
Plasmid#208033PurposeEnables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL45
Plasmid#174067PurposeExpresses CIB1 in S. cerevisiae. Includes inducible lac operon.DepositorInsertpGal1-LacO-3NLS-CIB1-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH126
Plasmid#172147PurposeMicroscopy inclusion body assayDepositorInsertpCDF.SUMO-B.(SD4).CD
UseTagsExpressionBacterialMutationPylBCD operon (SUMO tag inserted for PylB; RBS in…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE22
Plasmid#161787PurposeHi-SC2 reporter delivery plasmidDepositorInsertHi-SC2 reporter
UseConjugative plasmid to deliver hi-sc2 reporterTagsExpressionMutationHimar site included MmeI site, tandem loxP sites,…Promotern/aAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXF90
Plasmid#139044PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInserthalotag::HDEL (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY131
Plasmid#130956PurposeEngineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY76
Plasmid#130963PurposeEngineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY74
Plasmid#130945PurposeEngineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-G6
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY111
Plasmid#130942PurposeEngineered sgRNA-LEB4 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY110
Plasmid#130940PurposeEngineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY77
Plasmid#130964PurposeEngineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY124
Plasmid#130946PurposeEngineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter PbadDepositorInsertsgRNA-G6-2MS2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPbadAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY112
Plasmid#130944PurposeEngineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY107
Plasmid#130943PurposeEngineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY105
Plasmid#130939PurposeEngineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only