We narrowed to 10,315 results for: plasmids 101

1. p406MET25

   Plasmid

   #17421

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MET25 promoter is between SacI and XbaI restricti…

   Promoter

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. hACTB-RFP-Kir7.1

   Plasmid

   #107179

   Purpose

   Expression of N-terminal fused Kir7.1 in mammalian cells.

   Depositor

   Bikash Pattnaik

   Insert

   KCNJ13 (KCNJ13 Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   hACTB

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Mcm2-mCer

   Plasmid

   #211397

   Purpose

   mammalian expression of human Mcm2 C-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   Mcm2 (MCM2 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSyn-Aion-ts-mScarlet-ER

   Plasmid

   #192577

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   ts-mScarlet-ER

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMKII-Aion-ts-citrine-ER

   Plasmid

   #192576

   Purpose

   AAV-vector for optogenetic long-term silencing of excitatory neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   ts-Citrine-ER

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   CaMKIIa(0.4)

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-eGFP

   Plasmid

   #162823

   Purpose

   Doxycycline-inducible overexpression of eGFP

   Depositor

   Matthew Steinhauser

   Article

   Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V163A

   Promoter

   Tet ON

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCSG53_Nsp1

   Plasmid

   #167256

   Purpose

   Bacterial expression of Nsp1 residues 13-127

   Depositor

   Alexei Savchenko

   Article

   Semper et al iScience. 2021 Jan 22;24(1):101903. doi:

   Insert

   Nsp1 (residues 13-127) (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-TEV site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   April 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pZBTB11-donor

   Plasmid

   #112348

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZBTB11 with GFP

   Depositor

   Kevin White

   Insert

   ZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:3:101651181:A>T

   Promoter

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRR-PR-5Z

   Plasmid

   #23057

   Depositor

   Charles Miller

   Article

   Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

   Insert

   PR (PGR Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30del

    Plasmid

    #106902

    Purpose

    pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. punc-119c

    Plasmid

    #21595

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119 (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. punc-119cR

    Plasmid

    #21596

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119:mCherry (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    mCherry

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. Integrin-Alpha2-N-18

    Plasmid

    #58192

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: N/A, Emission: N/A

    Depositor

    Michael Davidson

    Insert

    Integrin-Alpha2 (ITGA2 Human)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. CACNA2D1-TwinStrepII

    Plasmid

    #83192

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    CACNA2D1 (Cacna2d1 Rat)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Casp2 C303A-Flag

    Plasmid

    #11812

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 catalytic mutant (CASP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C303A

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pET23b-Casp2-Delta Card

    Plasmid

    #11810

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 Delta Card (CASP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Delta Card

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pCMV6-XL4 FLAG-CBLN2-Fc

    Plasmid

    #115767

    Purpose

    Expression of the extracellular domain of CBLN2 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    CBLN2 (CBLN2 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKG187

    Plasmid

    #63077

    Purpose

    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIVTR(T7A)-ATOH1 (SA Substitutions)

    Plasmid

    #172309

    Purpose

    In vitro transcription of ATOH1 (Ser phosphosites substituted by Ala)

    Depositor

    Patrik Verstreken

    Insert

    ATOH1 (ATOH1 Human)
    

    Use

    Other

    Tags

    Expression

    Mutation

    S331A, S342A

    Promoter

    T7 promoter A-initiating

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLNCX1 Myr AKT2

    Plasmid

    #27294

    Depositor

    Morris Birnbaum

    Insert

    protein kinase B beta (AKT2 Human)
    

    Use

    Retroviral

    Tags

    HA and myristolation sequence

    Expression

    Mutation

    PH domain deleted (aa1-107)

    Promoter

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only