Addgene

Showing: 5981 - 6000 of 157741 results

Results per page:

Sort results by:

pDEST exorh:mGreenLantern

Plasmid

#195988

Purpose

Transgenesis backbone

Depositor

Nicolas Rohner, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-h53BP1 (siRNA resistant)

Plasmid

#110301

Purpose

Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

GAACGAGGA to GAGCGGGGC

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCINEO-3xFlag-hRIPK3

Plasmid

#112495

Purpose

Mammalian expression of 3xFlag-hRIPK3

Depositor

Junying Yuan

Insert

RIPK3 (RIPK3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Sac-Abeta(M1-40)

Plasmid

#71876

Purpose

Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-40) (APP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LeGO-C2

Plasmid

#27339

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-U6-sgRNA-EGFP

Plasmid

#107721

Purpose

For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-hHDAC3

Plasmid

#98591

Purpose

3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

Depositor

Zhaolan Zhou

Article

Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

Insert

dCAS9-HDAC3 (HDAC3 Human, Synthetic, S. pyogenes)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Flag Rac2

Plasmid

#12192

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

E-cadherin (Cdh1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY096-RetroEFS-CD22BBz-WPRE

Plasmid

#192197

Purpose

Retro-CD22CAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-CD22CAR (CD22 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW-Pol2-ffLuc2-eGFP

Plasmid

#71394

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

Pol2-ffLuc2-eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Pol2 (mouse RNA polymerase II gene promtoer)

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-Rab8a-wt

Plasmid

#86078

Purpose

A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_SFFv_4D5-High-CAR_RHL002

Plasmid

#164825

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Bmal1

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ

Plasmid

#206981

Purpose

empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Red Glifon Nega

Plasmid

#163117

Purpose

Red fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cells

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon Nega

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PER2:Luc Plasmid

Plasmid

#206875

Purpose

Per2 promoter luciferase reporter

Depositor

Michelle Farkas

Article

Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

Insert

Period 2 (Per2 Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLSL.R.Ly

Plasmid

#227697

Purpose

Reengineered geminiviral replicon vector

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge