164,826 results

1. pYSD063

   Plasmid

   #212916

   Purpose

   Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCR-YFP-GW

   Plasmid

   #209366

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis SCR promoter driving expression of N-terminal YFP fusion protein.

   Depositor

   Lucia Strader

   Article

   Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWOL-YFP-GW

   Plasmid

   #209365

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis WOL promoter driving expression of N-terminal YFP fusion protein.

   Depositor

   Lucia Strader

   Article

   Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-eIF4E-TwStrep

   Plasmid

   #208638

   Purpose

   for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

   Depositor

   Quanfu Ma

   Insert

   human eIF4E (ENST00000450253) (EIF4E Human)
   

   Use

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.4_1G11F2-3 heavy chain

   Plasmid

   #198253

   Purpose

   recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-HseIF4AI_T

   Plasmid

   #147543

   Purpose

   Insect Expression of HseIF4AI

   Depositor

   Elisa Izaurralde

   Insert

   HseIF4AI (EIF4A1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   3 silent mutations compared to the sequence given…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD649-HAsp-CEACAM1-COMP5AP-AviTag-9xHis

   Plasmid

   #157390

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CEACAM1 (CEACAM1 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCPP5371

   Plasmid

   #128732

   Purpose

   Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag

   Depositor

   Alan Collmer

   Article

   Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn1-mTurquoise2

   Plasmid

   #99125

   Purpose

   An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mTurquoise2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

    Plasmid

    #200641

    Purpose

    Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

    Depositor

    Fleur Ferguson, Justin English

    Article

    Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

    Insert

    miniTurboID-6xGGSG linker-FKBPF36V-2xHA
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. HDAC3 Flag

    Plasmid

    #13819

    Purpose

    Mammalian expression of human histone deacetylase 3 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC3 (HDAC3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-RUNX2

    Plasmid

    #192926

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX2 (RUNX2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+-mutTornado-split-nLuc

    Plasmid

    #212707

    Purpose

    Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    mutTornado-CVB3-split-nLuc
    

    Use

    Tags

    Expression

    Mutation

    Deleted the partial 3'ribozyme sequence in T…

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138132

    Purpose

    Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCas9-GFP

    Plasmid

    #78546

    Purpose

    Fusion protein Cas9+GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. ABE8e-Cas9NG

    Plasmid

    #203328

    Purpose

    Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    ABE8e-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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