We narrowed to 9,512 results for: try

1. pYPQ239A (dFnCas12a)-TV

   Plasmid

   #136379

   Purpose

   CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   dFnCas12a-TV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D917A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR4_EWSR1

   Plasmid

   #26367

   Depositor

   Thomas Tuschl

   Article

   Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Entry vector

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

3. p3E-EGFP-NS-MCS

   Plasmid

   #75180

   Purpose

   EGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   EGFP-NS-MCS
   

   Use

   Three prime entry vector for multisite gateway th…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVL23 - pVND7

   Plasmid

   #116019

   Purpose

   entry vector for GreenGate cloning method, promoter

   Depositor

   Thomas Greb

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pVND7
   

   Use

   Unspecified

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BC-A1-001

   Plasmid

   #78688

   Purpose

   A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. C413-E22: attB4-polyhedrinp>-attB1r

   Plasmid

   #162922

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-FRB-HA no-pA

   Plasmid

   #82594

   Purpose

   3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRB-HA (no pA)
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB1K3_pBen-sfGFP_J23101-BenR

   Plasmid

   #128125

   Purpose

   Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   sfGFP and BenR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBen and J23101

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP.R375

   Plasmid

   #138245

   Purpose

   Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSW177 - pCASP1

    Plasmid

    #115986

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Alexis Maizel

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pCASP1
    

    Use

    Unspecified

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ141-ZmUbi-RZ-Aa3.8

    Plasmid

    #136376

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. C455-E01: attB4-stuffer-attB5

    Plasmid

    #162983

    Purpose

    Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. C515-E01: attB5r-stuffer-attB1r

    Plasmid

    #162968

    Purpose

    Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH199_pE12_hyCal2-mCherry

    Plasmid

    #160412

    Purpose

    Gateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to mCherry

    Depositor

    Daniel Colón-Ramos

    Article

    Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

    Insert

    pDONR221 HyCal2::mCherry
    

    Use

    Other

    Tags

    mCherry

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. [GFP][IRES][Bla]

    Plasmid

    #97194

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAS-kkv-mos::NG

    Plasmid

    #138958

    Purpose

    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv (NEWENTRY Fly)
    

    Use

    Uas transgene vector

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Mutation

    8 bp that are in the region that encodes the cata…

    Promoter

    UAS

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. BC-A1-004

    Plasmid

    #78691

    Purpose

    A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
    

    Use

    Synthetic Biology

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BC-A1-003

    Plasmid

    #78690

    Purpose

    A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

    Plasmid

    #162958

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    triple FLAG epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-SGTAP no-pA

    Plasmid

    #82599

    Purpose

    3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    SBP-TEV-2x protein G
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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